R-Biopharm Rida Cycler, Руководство пользователя

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RIDA
®
CYCLER
Version 1.0
|
User manual
55
Multiple analyses are possible for each run, with the analysis being viewed highlighted blue on
the navigator bar.
Delete any analysis by selecting the Delete button next to the target name
12.1 Cycling analysis
Cycling Analysis allows you to determine the quantification cycle (Cq) and reaction efficiency
of each sample in your data set.
Cycling analysis is best used on its own when optimizing assays.
Cycling analysis is always provided with any other analysis type that uses cycling data
(Standard Curve and Absolute Quantification), allowing you to adjust parameters associated
with generating Cq and efficiency values.
By selecting Cycling Analysis, the software will, by default, plot baseline-corrected curves as
fluorescence (y-axis) against cycle number (x-axis), in logarithmic scale, for the target that was
chosen.
Fig. 49: Cycling Analysis window
12.1.1 Graph types
Derivatives: the first and second derivative curves for the selected data can be displayed in
parallel with baseline corrected amplification curves. The derivative curves can be used as a
reference to set an appropriate threshold or to gauge amplification quality.