R-Biopharm Rida Cycler, Руководство пользователя

Страница: 46 / 87

RIDA
®
CYCLER
Version 1.0
|
User manual
46
Unknown: Any sample that is under investigation.
Standard: A sample of known quantity, used to generate a standard curve from which
an unknown sample quantity can be calculated, or used to determine
amplification efficiency.
NTC:
A sample that contains no template. NTC’s are used to monitor for
amplicon contamination in the reaction and for the amplification of non-
specific amplicons (primer dimers) when using intercalating dye
chemistries.
Positive: The sample is known to contain the target of interest. A positive control is
used to confirm that the assay is working and helps prevent false
negatives.
Negative: The sample is known not to contain the target of interest. A negative
control is used to monitor for contamination of the assay and is helpful in
preventing false positives. Unlike the NTC, the negative control can
contain an internal amplification control template to ensure the PCR is
working.
9.1.6 Sample Concentrations
Select the units to report.
There is a list of units to choose from. Alternatively, enter your own units, in the provided text
box.
Enter a Concentration for each standard.
When using standards, it is a requirement to provide a value for each one. The value can be a
quantifiable absolute or an arbitrary number. Numbers can also be entered in scientific
notation (1E03 = 1 x 103 = 1000)
Enter the values one at a time or use the Auto fill option to quickly add a serial dilution and
replicates by doing the following:
Enter the first concentration value
(20,000).
Leave the same number of rows blank
as the number of replicates required,
below the first concentration. Enter the
second concentration in the dilution
series (10,000).