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Bioscience Solutions
– 4D-Nucleofector™ Manual
1 The Nucleofector™ Technology
Since its introduction in 2001, Nucleofector™ Technology has transformed
transfection. Nucleofection has proven to be a reliable and reproducible
easy-to-use technology, suited to a wide variety of applications. An ever
increasing number of publications in a wide range of research areas reflect
how Nucleofector™ Technology is driving research in numerous cell types
and applications. Nucleofector™ Technology provides an answer to almost
every transfection challenge.
How it Works
Nucleofector™ Technology is based on two unique components, the
Nucleofector™ System that delivers the specifically optimized electrical
parameters and Nucleofector™ Kits, which contain specific Nucleofector™
Solutions. As part of Nucleofector™ Technology, Lonza provides cell-type
specific optimized protocols for many different cell lines and primary cells.
Transfection of Any Cell
With over 160 protocols optimized by Lonza’s R&D Team for cell lines and
primary cells and more than 680 entries in the online Cell Transfection
Database, Nucleofector™ Technology is the transfection method of choice
for difficult-to-transfect cell types. Moreover, Nucleofection is the only
electroporation-based technology allowing reliable transfection of
adherent cells and cells in suspension.
Transfection of Any Substrate
Nucleofector™ Technology offers high flexibility within applications,
since the same transfection parameters apply for almost all substrates.
DNA vectors, RNA duplexes, and peptides can be transfected using our
sophisticated transfection protocols. This makes Nucleofection an ideal
tool for providing answers to scientific questions in over-expression
studies, gene silencing approaches, protein expression, generation of
stable clones and many more applications besides.
Moving Transfection into the Forth Dimension
At Lonza, we believe that listening to our customers is the best way
to develop innovative products that meet your needs. In line with this
customer-oriented spirit, the 4D-Nucleofector™ System was developed
to offer advanced performance, flexibility and convenience. The system
is controlled by intuitive software on an up-to-date touch screen user
interface. Its modular architecture allows seamless expansion of the
system for new applications.
Experience the Advanced Nucleofector™ Platform:
– A novel conductive polymer based cuvette system completely free
of toxic metal ions
– Transfection of different cell numbers using the same conditions
– Nucleofection of cells in adherence
– Closed, scalable transfection of larger cell numbers in the range of
1x10
7
to 1x10
9
