Lonza 4D-Nucleofector, Руководство пользователя

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4D-Nucleofector™ Manual – Bioscience Solutions 21
2.11.1 Using the 1 mL Nucleocuvette™ Cartridge
(Fixed Volume)
2.11.1.1 Defining a New Experiment
1. After unit and vessel type selection (see chapter 2.8.2) a screen
appears which allows you to define experiment parameters select a
previously saved experiment by pressing “Choose existing experiment”
(figure 2.20, A).
2. Press the field “CELL TYPE PROGRAM” to choose predefined
Nucleofection conditions from a cell type list (figure 2.20, B). Use
the search (magnifying glass symbol) or the sort list functions (A-Z)
to find conditions more quickly.
a. Select the desired cell type code by tapping on the appropriate
line of the cell list. The cell type selected will be highlighted. For
additional information about the cell type selected press “i”.
b. To confirm your selection press “OK”.
c. If required, modify pulse code by pressing the letter or number
code fields. A keyboard will appear, enabling you to change
settings. The solution code can be modified via a selection list.
3. Instead of defining solution and program code via the CELL TYPE
PROGRAM, both parameters can also be selected manually, e.g. in
case no predefined cell type program is available. For adding new cell
type programs, please refer to chapter 2.12.3.
4. Optional: At this stage (or at step 8) you can save your defined
experiment for future use by pressing the “SAVE” button
(figure 2.20, C). A keyboard will appear allowing you to define a name
(max. length: 26 characters).
5. You may enter further information about your experiment by touching
the “Info” field and typing in your text (figure 2.20, D).
6. Confirm and save the experiment parameters by pressing “OK” or
“SAVE”.
7. Press OK to continue (figure 2.20, E)
8. A summary of the defined settings will be displayed (figure
2.20, F). Please check for correctness before continuing.
2.11.1.2 Loading Samples
9. Fill the 1 mL Nucleocuvette™ Cartridge with the cell-substrate solution
(figure 2.21, A ). A volume of 1 mL is required to completely fill the
cuvette. Using a higher or lower volume may lead to errors.
10. Insert the filled cartridge into the slot until it snaps in (figure 2.21, B).
C D
E F
A B
Figure 2.20: Experiment definition (LV Unit, 1 mL Nucleocu-
vette™ Cartridge)
Figure 2.21: Loading of sample (LV Unit, 1 mL Nucleocuvette™
Cartridge)
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