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4D-Nucleofector™ Manual – 

Bioscience Solutions

   37

5  Purchaser Notification

Purchaser Notification

The purchase of the 4D-Nucleofector™ System (the “Purchased Device”) conveys to the buyer 
a limited non-transferable, non-exclusive license (the “License”) for the use of the Purchased 
Device and Lonza’s proprietary Nucleofector™ process and methods (the “Licensed Process”) 
without the right to sublicense.

The License is limited to research use only. The License does not include the right to use the 
Purchased Device, its components or materials, or the Licensed Process for any Commercial 
Purpose, as defined below. Notwithstanding the foregoing, the License permits the buyer 
to use the Purchased Device in combination with the Licensed Process to perform services 
internally for affiliates, subsidiaries, sections, departments or scientific collaborators, pro-
vided that such services are not for any Commercial Purpose.

Under no circumstances shall the Purchased Device or the Licensed Process be used for 
testing or treatment in humans. The buyer, through the purchase of the Purchased Device, 
acknowledges that the Purchased Device and Licensed Process are not diagnostic or thera-
peutic tools and shall not be used as an accessory or complement to such tools.

“Commercial Purposes”, as used herein, means the sale, license, lease, export, transfer or 
other distribution of the Purchased Device, its components or materials, or the Licensed 
Process to a person or entity other than the buyer for financial gain or other commercial 
purposes and/or the use of the Purchased Device, its components or materials, or the Li-
censed Process: (1) in manufacturing or producing a product for general sale or products 
for use in the manufacture of products ultimately intended for general sale; (2) to provide 
a service, information, or data to a person or entity other than the buyer for financial gain; 
or (3) for therapeutic, diagnostic or prophylactic purposes. 

The License does not permit the buyer to manufacture, copy, reproduce, transmit, distrib-
ute, sell, lease, transfer or sublicense the Licensed Process and/or the Purchased Device.
For information on obtaining a license to the Purchased Device or the Licensed Process for 
purposes other than research, contact our Licensing Department, at +49 221 99199 0 or 
e-mail: [email protected]

Representations and Warranties

Lonza represents and warrants that, it is the holder of intellectual property, including patents, 
patent applications, copyrights, trademarks and technical and scientific knowledge with 
respect to the Purchased Device and the Licensed Process and that, to its knowledge, it has 
the right to grant the License provided herein. Lonza further represents and warrants that 
it has no knowledge of any rights of third parties which supersede its intellectual property 
rights to the Purchased Device and the Licensed Process and that Lonza has full corporate 
power and authority to grant the License provided hereunder.

Lonza does not warrant that the information and data pertaining to the Licensed Process 
and/or the Purchased Device is correct and without defects, that the use of such informa-
tion or data is adequate for the use of the Licensed Process and/or the Purchased Device 
or that the technical information or data for the Licensed Process and/or the Purchased 
Device is complete.

Lonza does not warrant either that the use of the License does not infringe third parties’ 
rights or does not cause damages to third parties. Any liability for later invalidation or lapse 
of Lonza’s intellectual property is excluded.

THE EXPRESS WARRANTY PROVIDED ABOVE IS LONZA’S ONLY WARRANTY RELATING TO THE 
PURCHASED DEVICE OR THE LICENSED PROCESS AND IS IN PLACE OF ALL OTHER WARRANTIES, 
EXPRESSED OR IMPLIED, STATUTORY OR OTHERWISE, INCLUDING WARRANTIES AS TO MERCHANT-
ABILITY OR FITNESS FOR A PARTICULAR PURPOSE, ALL OF WHICH ARE EXPRESSLY EXCLUDED. 
BUYER AGREES THAT LONZA HAS MADE NO UNDERTAKINGS, AGREEMENTS OR REPRESENTA-
TIONS, EXPRESSED OR IMPLIED WITH RESPECT TO THE PURCHASED DEVICE OR THE LICENSED 
PROCESS, OTHER THAN THOSE EXPRESSLY SET FORTH HEREIN. 

NOTWITHSTANDING ANYTHING TO THE CONTRARY SET FORTH HEREIN, IN NO EVENT WILL LONZA 
BE LIABLE FOR LOST PROFITS, DAMAGE TO REPUTATION, OR LOSS OF DATA, OR FOR ANY SPECIAL, 
INDIRECT, INCIDENTAL, PUNITIVE, OR CONSEQUENTIAL DAMAGES, HOWEVER CAUSED, ON ANY 
THEORY OF LIABILITY AND WHETHER OR NOT LONZA HAS BEEN ADVISED OF THE POSSIBIL-
ITY OF SUCH DAMAGES, ARISING UNDER ANY CAUSE OF ACTION AND ARISING IN ANY WAY OUT 
OF BUYER’S USE OF THE PURCHASED DEVICE OR THE LICENSED PROCESS. BUYER HEREBY 
AGREES THAT TO THE FULLEST EXTENT PERMITTED BY LAW, LONZA’S LIABILITY TO BUYER FOR 
ANY AND ALL INJURIES, CLAIMS, LOSSES, EXPENSES, OR DAMAGES WHATSOEVER, ARISING 
UNDER ANY CAUSE OF ACTION AND ARISING IN ANY WAY OUT OF BUYER’S PURCHASE OR USE 
OF THE PURCHASED DEVICE OR LICENSED PROCESS SHALL BE LIMITED IN THE AGGREGATE, 
IN LONZA’S SOLE AND ABSOLUTE DISCRETION, TO REPAIR, REPLACEMENT, OR REFUND OF THE 
EQUIPMENT OR EQUIPMENT PART, AS APPLICABLE, OR THAT PORTION OF THE PURCHASE PRICE 
APPLICABLE TO THE EQUIPMENT OR THE EQUIPMENT PART, AS APPLICABLE, WHICH VIOLATES 
AN EXPRESS WARRANTY PROVIDED HEREUNDER. NOTWITHSTANDING ANYTHING HEREIN TO THE 
CONTRARY, THE LIABILITY OF LONZA WITH RESPECT TO A CLAIM OF ANY KIND WHATSOEVER, 
SHALL NOT EXCEED THE INVOICE PRICE OF THE PARTS, REPLACEMENT PARTS OR SERVICE AS 
TO WHICH THE CLAIM IS MADE. 

Buyer assumes all responsibility and liability for injury or damages resulting from its hand-
ling, possession, use or sale of the Purchased Device or Licensed Process including, but not 
limited to any injury or damage resulting from the use of the Purchased Device or Licensed 
Process in Buyer’s operations or in combination with other substances, materials or products. 
Buyer agrees to defend, indemnify and hold Lonza and its affiliates and their respective 
directors, officers, employees, agents harmLess from and against all claims, losses, dam-
ages, liabilities and expenses (including attorneys’ fees and other litigation or settlement 
costs) arising out of (a) Buyer’s handling, possession, use or sale of the Purchased Device 
or Licensed Process, (b) any material breach of any Agreement between Lonza and Buyer or 
other terms and conditions relating to the use of the Purchased Device or Licensed Process 
provided to Buyer by Lonza, or (c) the gross negligence or willful misconduct of Buyer or any 
of its affiliates in performing any activity relating to the subject matter hereof.
Lonza does not assume any liability that the Licensed Process, the Purchased Device or 
any product resulting from the use of the Licensed Process or use of the Purchased Device 
shall be approved by governmental authorities or any other institution or supervisory board 
or similar agencies.

5

Содержание 4D-Nucleofector

Страница 1: ...Bioscience Solutions 4D Nucleofector System Manual For Research Use Only...

Страница 2: ...System conveys to the buyer the non transferable right to use the system as well as Lonza s proprietary Nucleofector Technology for research conducted by the buyer whether the buyer is an academic or...

Страница 3: ...ofector Y Unit 18 2 10 1 Defining a New Experiment 18 2 10 2 Loading Samples 19 2 10 3 Running the Experiment 19 2 11 4D Nucleofector LV Unit 20 2 11 1 Using the 1 mL Nucleocuvette Cartridge Fixed Vol...

Страница 4: ...owing reliable transfection of adherent cells and cells in suspension Transfection of Any Substrate Nucleofector Technology offers high flexibility within applications since the same transfection para...

Страница 5: ...ioscience Solutions 5 The 4D Nucleofector System Support Teams Europe Scientific Support 32 87 321 611 scientific support eu lonza com North America Scientific Support 1 800 521 0390 toll free scienti...

Страница 6: ...power supply before cleaning and disinfecting the case Useadampclothtowipedowntheoutercasewithwateror70 80 ethanol Do not use any aerosols for cleaning Avoid wetting the cuvette holder within the cuv...

Страница 7: ...nit 1 mL or LV Nucleocuvette Cartridge for LV Unit 24 well Dipping Electrode Array forYUnit UsingconsumablesfromanyothersourcethanLonzawill preclude all warranty and liability claims Do not alter the...

Страница 8: ...mple rescue also see 3 2 using wheel accessible after removal of side panel via screw behind termination plug A typical 4D Nucleofector System includes 1 One 4D Nucleofector Core Unit 2 At least one f...

Страница 9: ...for completeness 2 Stack the units with the Core Unit containing the touch screen on top figure 2 4 3 Connect the units figure 2 5 by using the interface cables Connect the interface outlet port of t...

Страница 10: ...ector System Core Unit and functional units Figure 2 6 Main screen 2 7 2 Adjusting the Position of the Touch Screen The touch screen of the 4D Nucleofector System can be set at four angles 0 30 45 and...

Страница 11: ...onfirm selection or execute a program A Z Z A Top 10 Last 10 Sort a list alphabetically display the most frequent 10 items or the most recent 10 items Magnifier symbol Activate search functions i Disp...

Страница 12: ...s Load Samples chapter 2 9 2 10 or 2 11 Execute Nucleofection Experiment chapter 2 9 2 10 or 2 11 Prepare Samples According to Cell type Specific Optimized Protocol 2 8 General Instructions for Runnin...

Страница 13: ...ntaining cells and substrate The selected Nucleofection program will be applied to this position 2 No DNA Negative control Nucleofection program applied to vessel with cells but without substrate 3 No...

Страница 14: ...line of the list The experiment selected will be highlighted Confirm your selection by pressing OK 5 By pressing on the icons displaying the Nucleofection vessels or wells you can check the settings...

Страница 15: ...Z to find conditions more quickly a Select the desired cell type code by tapping on the appropriate line of the cell list The cell type selected will be highlighted For additional information about t...

Страница 16: ...leocuvette Strip theyellowpinattherearend of thestripmustbevisible figure2 14 C Ifthestripismounted inthewrongorientationitsrearendwillstayabovethestripholder and the yellow pin is hardly visible figu...

Страница 17: ...te s Proceed willbe displayed figure 2 15 C Load next samples and press YES to continue or press NO to interrupt the experiment 12 When the experiment is complete a result file summarizing the Nucleof...

Страница 18: ...line of the cell list The cell type selected will be highlighted For additional information about the cell type selected press i b To confirm your selection press OK c If required modify pulse code b...

Страница 19: ...the softwarewillcheckforusedwellsandofferyoudifferentoptionshow to continue 2 10 3 Running the Experiment 12 Afterloadingthesamplespress START toruntheexperiment figure 2 18 A 13 The progress of the...

Страница 20: ...part area 3 Injection port rear side 4 Air outlet port with 0 2 M sterile filter Components of the LV Nucleocuvette Cartridge figure 2 19 B C 1 Cartridge 2 Venting tube with 0 2 M sterile filter 3 Inl...

Страница 21: ...of defining solution and program code via the CELL TYPE PROGRAM both parameters can also be selected manually e g in casenopredefinedcelltypeprogramisavailable Foraddingnewcell type programs please r...

Страница 22: ...y a color code green for OK figure 2 22 C yellow in case of few errors and red in case of multiple errors figure 2 22 D If errors occurred you can check for more details by pressing More details There...

Страница 23: ...KIP and continue with step 14 4 Upon pressing YES the pinch valves will automatically open for 30 secondsallowingtoconvenientlyinsertthetubesintothethreepinch valves and a new window will appear figur...

Страница 24: ...o cell type specific protocol and mount it into a 4D Nucleofector LV Reservoir Rack figure 2 23 I 12 RemovetheredcapfromtheSpirosconnectorontheoutlettubeofthe cartridge and connect it to the swabbable...

Страница 25: ...apter 2 12 3 17 Optional At this stage or at step 20 you can save your defined experimentforfutureusebypressingthe SAVE button figure2 24 D Akeyboardwillappearallowingyoutodefineaname max length 26 ch...

Страница 26: ...pressing START H I Figure 2 25 Setup Checklist for LV Nucleocuvette Cartridge 1 Cartridge inserted in slot 2 Short venting tube with filter attached to the male Luer connector on the front plate 3 Tub...

Страница 27: ...rs figure 2 26 E and red in case of multiple errors figure 2 26 G If errors occurred you can check for more details by pressing More details The result details figure 2 26 F H will display the type of...

Страница 28: ...ttings list Firmware update update the system software see chapter 2 12 7for details Device cleaning opens the tray of the functional modules to remove it for cleaning see chapter 2 12 5 for details S...

Страница 29: ...he NEW field Edit existing programs by pressing EDIT or save your programs onto a USB stick by pressing SAVE figure 2 27 E To create a new custom program or to edit an existing program Press NEW or se...

Страница 30: ...D Nucleofector SystemwiththePCEditorsoftware seechapter2 13 Itisnecessaryto perform synchronization with each firmware update To start synchronization Insert a USB stick into the USB port on the front...

Страница 31: ...drive Insert 4D Nucleofector user information USB stick into the USB port of your PC Copy all files from the 4D Nucleofector PC Editor software folder to your hard disk preferably into a folder calle...

Страница 32: ...tion figure 2 29 B Select the USB stick and press SAVE Unplug the USB stick from your PC as soon as the experiment file has been saved NOTE Do not unplug the stick while saving Turn on the 4D Nucleofe...

Страница 33: ...easing DNA amount may lead to higher transfection efficiency but at the same time result in higher cell mortality Cell number in Nucleofection sample too high or too low Please use the cell numbers re...

Страница 34: ...irection of the red arrow as far as it will go figure 3 1 B Model variant B Newer Unplug system from main power Remove the terminator cap or cable connection to next unit figure 3 1 C Insert screw dri...

Страница 35: ...argecircumventingthesampleandis oftenaccompaniedbyaflashandanoise Thisproblemisusuallycaused by imperfect cuvettes or cuvette filling The 4D Nucleofector System is equipped with a hardware safety feat...

Страница 36: ...e of dipping electrodes that had been used before Err 22 Used well A weak pulse occurred Efficiency may be sub optimal Re used dipping electrode or inappropriate Nucleofector Solution Clear the error...

Страница 37: ...a for the Licensed Process and or the Purchased Device is complete Lonza does not warrant either that the use of the License does not infringe third parties rights or does not cause damages to third p...

Страница 38: ...n interfaces interface RS422 USB Lonza interface for module connection Shuttle HV Out X Unit only Spannungsbegrenzung voltage limitation limitation de tension 1500 V Strombegrenzung current limitation...

Страница 39: ...4D Nucleofector Manual Bioscience Solutions 39 6...

Страница 40: ...partyowners Theinformationcontainedhereinisbelievedtobecorrect and corresponds to the latest state of scientific and technical knowledge However nowarrantyismade eitherexpressedorimplied regarding it...

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