24
Bioscience Solutions
– 4D-Nucleofector™ Manual
9. Insert the upper tube into the upper pump and close the flap (figure
2.23, H).
10. Repeat with the lower tube and the lower peristaltic pump (optional if
working with 2 reservoirs).
11. Fill
outlet reservoir
with medium (for detailed recommendations,
please refer to cell type-specific protocol) and mount it into a
4D-Nucleofector™ LV Reservoir Rack (figure 2.23, I).
12. Remove the red cap from the Spiros connector on the outlet tube of the
cartridge and connect it to the swabbable injection port of the outlet
reservoir (or any other reservoir with female Luer connector, figure
2.23, J).
13. Before continuing with defining the experiment (chapter 2.11.2.2)
check setup status. At this point steps 1-6 and 11 of the arrangement
shown in figure 2.25 should be done.
H
I
J
Figure 2.23:
Continued
