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Bioscience Solutions
– 4D-Nucleofector™ Manual
2.11.1.3 Running the Experiment
11. When the cartridge is inserted, press “START” to initiate the
Nucleofection process (figure 2.22, A).
12. The progress of the experiment is indicated by a progress bar (figure
2.22, B).
13. When the experiment is complete, a result file summarizing the
Nucleofection process will be displayed.
14. The result file will be saved automatically by the system and can
be reopened as described in chapter 2.12.2. The overall result is
indicated by a color code: green for “OK” (figure 2.22, C), yellow in
case of few errors and red in case of multiple errors (figure 2.22, D).
If errors occurred you can check for more details by pressing “More
details”. The result details (figure 2.22, E) will display the type of error
and its percent occurrence in the processed volume. For additional
information on the error press “i”.
15. Once the process is completed take out the cartridge by pressing the
black levers.
16. Aspirate the transfected cell suspension and transfer cells into culture.
Optionally you may wash the cartridge with medium to aspirate
residual liquid.
17. You can repeat the same experiment by pressing “START” again.
C
E
D
A
B
Figure 2.22:
Experiment progress (LV Unit, 1 mL Nucleocuvette™ Cartridge)
