4D-Nucleofector™ Manual –
Bioscience Solutions
13
C
B
A
Parameter
Description
Cell Type Program
Using the “Cell Type Program” option is the easiest way to select parameters for a vessel or well or group of those.
Each “Cell Type Program” is assigned to the optimal program for the certain cell type as defined in the Optimized Protocol
(e.g., “T cells, human, unstim.” Is affiliated to the optimal Nucleofector™ Program and Solution for unstimulated human T
cells).
The “Cell Type Program” list can be extended by the user with customized cell-type codes (e.g., after a cell line optimization;
2.12.3). Lonza defined programs are highlighted in blue while custom program codes will be highlighted in black.
Solution
In case the recommended Nucleofector™ Solution is not selected automatically via the “Cell Type Program” option, the
“Solution” can be selected manually.
Pulse Code
In case the recommended Nucleofector™ Program is not selected automatically via the “Cell Type Program” option, a “Pulse
Code” (e.g. FI-115) can be entered manually.
Note:
For manual “Pulse Code” selection, the “Solution” has to be selected first (see below).
Control
(X and Y Unit)
1. Sample: This is the default setting and defines a normal sample containing cells and substrate. The selected
Nucleofection program will be applied to this position.
2. No DNA: Negative control. Nucleofection program applied to vessel with cells but without substrate
3. No program: Negative control. No Nucleofection program applied to a vessel containing cells and substrate
In case of “Sample” or “No DNA” the selection has only an informative purpose. The selection of “No program” has a
functional effect, i.e. no pulse will be applied.
Volume (LV Unit with
LV Nucleocuvette™
Cartridge)
When using the scalable cuvette version, the LV Nucleocuvette™ Cartridge, the volume that should be processed has to be
defined. Depending on the number of reservoirs used, one or two volume parameters must be specified. The maximum total
volume that can be entered is 20 mL.
2.8.2
Unit and Vessel Selection
1. Select the functional unit (X, Y or LV) you wish to work with by pressing
the appropriate icon (figure 2.10, A).
2. For X and LV Unit:
A screen appears displaying the Nucleofection
vessels available for the selected functional unit (figure 2.10, B, C).
Press the icon displaying the vessel type you wish to use.
2.8.3
Experiment Parameters - Overview
An experiment comprises a selected set of vessels or wells that should
be processed with defined Nucleofection parameters. Table 2.3 describes
the parameters that can be defined.
Figure 2.10:
Unit and vessel selection
Table 2.3:
Unit and vessel selection
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