4D-Nucleofector™ Manual –
Bioscience Solutions
23
2.11.2
Using the LV Nucleocuvette™ Cartridge
(Scalable Volume)
Before starting to prepare any cells
– Insert LV Nucleocuvette™ Cartridge including all tubes into the
4D-Nucleofector™ LV Unit (see steps 1-10 below)
– Connect outlet reservoir (see steps 11-12 below)
– Define the experiment (chapter 2.11.2.2)
2.11.2.1 Mounting an LV Nucleocuvette™ Cartridge
1. Insert the pre-assembled LV Nucleocuvette™ Cartridge into the slot
until upper and lower snap mechanism audibly snaps in.
2. Attach the short venting tube with the filter (upper left of the cartridge)
to the male Luer connector on the front plate (figure 2.23, A).
3. After unit and vessel type selection (see chapter 2.8.2) a pop
up window will appear asking whether you want to insert the
pre-assembled LV Nucleocuvette™ Cartridge the device now
(figure 2.23, B). If you want to do that at a later time point press “SKIP”
and continue with step 14.
4. Upon pressing “YES” the pinch valves will automatically open for 30
seconds allowing to conveniently insert the tubes into the three pinch
valves and a new window will appear (figure 2.23, C).
Note:
The open time of the pinch valves is limited to 30 seconds to avoid
overloading of the electronics. Alternatively, you can open the
pinch valves manually by pushing the black button on the valves.
5. Insert the tubes into the three pinch valves (figure 2.23, D). Ensure
that they are inserted completely (figure 2.23, E). After 30 seconds,
the pinch valves will close automatically.
6. Press “DONE” when mounting is completed (figure 2.23, C). In case
you did not finalize insertion within 30 seconds, you may re-open the
valves by pressing “YES” again or via “Settings” menu or manually
(see “Note” above).
7. Insert the T connector on the left hand side of the cartridge into the T
connector holder (figure 2.23, F).
8. Insert the two larger diameter tubes into the liquid sensors (figure
2.23, G). Ensure the tubes are inserted completely into the sensors.
B
C
D
E
A
Figure 2.23:
Mounting the LV Nucleocuvette™ Cartridge
F
G
2
