11 Use a syringe to remove the remaining hydraulic liquid above the adapter in the
column.
NOTICE
Always wear gloves when handling the adapter rod to avoid
fat from unprotected fingers and scratching the sealing surface.
12 Rinse the space above the adapter with distilled water, remove with a syringe and
discard.
13 Carefully lower the top plate and close it using the bayonet tools.
14 Pump the adapter to its upper position. A sudden pressure drop indicates that the
adapter has passed the beveling at the top of the (glass) tube. By then the adapter
has come loose from the column tube and can be removed (Removing the top plate
and adapter, on page 49).
CAUTION
When handling the adapter and top plate assembly, ensure that
fingers or hands are not caught in between the top plate and the
adapter when the top plate slides down the adapter rod.
Limitation of adapter movement in the long column tube
The length of the adapter rod in a long column tube is designed for packing bed heights
from 30 cm to 50 cm. The adapter rod length is insufficient to allow adapter movement
much below a 30 cm packing height. If the adapter moves too far into the column the
pressure is automatically reduced in the hydraulic chamber thus preventing further
downward movement of the adapter. This reduction in pressure is facilitated by a groove
at the top of the adapter rod, which when entering the column through the top plate of
the column allows a leakage through the O-rings at the groove. If the leakage occurs,
move the adapter upwards in the column until the groove is visible outside the column
and the hydraulic chamber will become sealed once again.
CAUTION
Risk of leakage. Take proper precautions for personal protection
and surrounding equipment.
48
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
4 Getting started
4.6 Routine procedures
