Table of Contents
5
1
Introduction ..........................................................................................................
5
1.1
General introduction ...........................................................................................................................
5
1.2
Scope of this Operating Instructions ...........................................................................................
6
1.3
Important user information .............................................................................................................
8
1.4
Regulatory information ......................................................................................................................
10
2
Safety instructions ...............................................................................................
10
2.1
Safety precautions ...............................................................................................................................
14
2.2
Column pressure and over-pressure ...........................................................................................
15
2.3
Labels .........................................................................................................................................................
17
3
Specifications and characteristics ....................................................................
17
3.1
Principles of column function .........................................................................................................
22
3.2
Primary specifications ........................................................................................................................
23
3.3
Column lengths and bed support materials ............................................................................
23
3.4
Materials ...................................................................................................................................................
26
3.5
Chemical resistance ............................................................................................................................
29
3.6
Packing flow rates ................................................................................................................................
30
4
Getting started .....................................................................................................
30
4.1
Checking the packing list ..................................................................................................................
31
4.2
Identifying the main components of the column ..................................................................
32
4.3
Bed supports ...........................................................................................................................................
34
4.4
Wetting instructions for plastic bed supports .........................................................................
35
4.5
Other equipment ...................................................................................................................................
36
4.6
Routine procedures .............................................................................................................................
50
4.7
Over-pressure situation .....................................................................................................................
53
4.8
Preparing the column for a run ......................................................................................................
57
4.9
Installation in basic configuration ................................................................................................
60
4.10
Installing the UNICORN Intelligent Packing strategy on ÄKTAexplorer and ÄKTApi-
lot .................................................................................................................................................................
61
4.11
Selecting the Intelligent Packing strategy and component ..............................................
62
5
Operation ..............................................................................................................
63
5.1
Intelligent Packing of the column with ÄKTAexplorer and ÄKTApilot ...........................
79
5.2
Intelligent packing of the column with ÄKTA avant 150 .....................................................
80
5.2.1
Create a column type .......................................................................................................................
84
5.2.2
Creating an Intelligent Packing method using ÄKTA avant 150 ....................................
93
5.2.3
Preparing the slurry, priming and packing the column using ÄKTAavant ................
94
5.3
Manual packing of the column ......................................................................................................
100
5.4
Performance evaluation of the column .....................................................................................
102
5.5
Unpacking a packed column ..........................................................................................................
103
5.6
Cleaning-in-place (CIP) ......................................................................................................................
103
5.7
Sanitization ..............................................................................................................................................
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
3
Table of Contents
