1
Disconnect the tubing from the system leading to the mobile phase at the adapter
rod top [105] and fit a stop plug onto the tubing.
2
Ensure that the pocket at the arm of the mechanical locking equipment is properly
positioned at the adapter rod top. Check that the tie rods of the mechanical locking
are screwed tight into the arm.
If the mechanical locking equipment is correctly fitted there should be no
thread visible on the tie bars under the locking arm.
Note:
3
Remove the stop plug from the tubing and connect to the system again.
4
Run the locking nuts up the thread of the tie rods until in contact with the top bayonet,
tighten loosely by hand.
NOTICE
By tightening the locking nuts on the mechanical locking
equipment it is possible to work the adapter down and to
compress the bed further than what was intended during the
packing of the column.
5
Check that the mechanical locking equipment is firmly fixed onto the column and
that the parts sit securely.
6
To avoid air entering the column after disconnection/ connection to the system,
apply upflow through the column to evacuate any air trapped in the tubing.
7
Insert and hold a syringe into the top of the hydraulic chamber vent valve to avoid
liquid spraying out from the hydraulic chamber . Relieve the pressure in the hydraulic
chamber by opening the hydraulic chamber vent valve [107] at the top plate [803].
(Releasing pressure from the hydraulic chamber, on page 51).
CAUTION
If the hydraulic chamber vent valve is opened while there is
pressure in the hydraulic chamber, liquid will spray out. Take
necessary precautions to avoid personal injury or damage to
equipment.
40
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
4 Getting started
4.6 Routine procedures
