12 Monitor the pressure on the pressure gauge. The pressure drop should not exceed
0.1 bar g during 5 minutes. A recorder linked to a pressure controller gives more
precise figures during this procedure.
13 If the pressure drop exceeds this limit try to localize the source of the leakage. Begin
with the valves and outlets for signs of leakage. If the leakage cannot be localized
there, then the adapter sealing system is most likely the cause. Complete the test,
empty the column, and take the necessary steps to rectify the suspected problem.
Repeat the leakage test.
Leakage test for the process
chamber
1
Fill the column with liquid and assemble the adapter and top plate assembly onto
the column (see Fitting the top plate and adapter, on page 41).
2
Ensure that one mobile phase inlet/outlet is open and the other closed.
3
Insert a syringe into the vent valve and open the valve.
4
Pump liquid into the hydraulic chamber through the hydraulic chamber inlet.
5
Close the hydraulic chamber vent valve when only liquid escapes the valve.
6
The adapter moves down. Stop the pump when the adapter is at a suitable height.
Water should emerge from the open mobile phase inlet/outlet.
7
Assemble the mechanical locking equipment (see Mechanical locking, on page 39).
8
Pump liquid into the process chamber at a low liquid velocity while monitoring the
pressure. Stop the pump when pressure is equal to maximum packing pressure (see
Section 3.2 Primary specifications, on page 22).
In order for the pressure to continue to be registered on an ÄKTA system
when the pump has stopped, the flow must be set to zero (Flow = 0). If
Pause or Stop are used to stop the flow, this will even cause the registration
of pressure to cease.
Note:
9
Monitor the pressure on the pressure gauge. The pressure drop should not exceed
0.1 bar g during 5 minutes. A recorder linked to a pressure controller gives more
precise figures during this procedure.
10 If the pressure drop exceeds this limit try to localize the source of the leakage. Begin
with the valves and outlets for signs of leakage. If the leakage cannot be localized
there, then the adapter sealing system is most likely the cause. Complete the test,
empty the column, and take the necessary steps to rectify the suspected problem.
Repeat the leakage test.
144
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
6 Maintenance and service
6.4 Performing a column leakage test
