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ROCK IMAGER User's Guide | Chapter 13: Improving Images
e. Return to the Plate Type Editor window, and click the
button next to the
well location to copy the coordinates.
f. Repeat steps d and e for configuring the other three corner wells of the plate.
5. Verify the well positions.
a. Return to the
Imager
tab.
b. Under the
Well Control
, select
Well
, type a well location in the Well box (such as
A1
), and choose a
Drop
option from the corresponding list.
c. Click the
C
button. ROCK IMAGER should display the center of the selected well
drop. You can move to the next well drop by clicking the arrow buttons on the
well control pad.
6. Return to the Plate Type Editor window. Specify the
Drop Location
and the
Method
that
you would like to use.
Fixed Drop Location
should be used for drops that are centered in
the well with consistent relative positions. This method will have the fastest imaging time
and is recommended for routine sitting drop experiments.
Standard Drop Location
should
be used for soluble protein experiments and is useful for plates where the drop position is
not consistent and the size of the drops are small relative to the well area.
7. When done, click
Apply
.
8. Click the
Export to File
button in the lower left corner of the Plate Type Editor window, and
then browse the desired location to save your new plate definition.
Plate masks are used to hide autoflorescence that occurs in certain plate types for more accurate
Auto Scoring results. Although it is called a plate mask, you will set the mask using a particular drop
on the plate. Once you set the plate mask for a single drop, the mask will apply to all drop locations
on the plate — hence the name plate mask.
Drop A1
Applying Plate Masks
