76
Drop Location
settings determine how ROCK IMAGER executes the
Drop Location
algorithm.
Selecting
Fixed
instructs ROCK IMAGER to use the drop positions defined in the
Drop Positions
field
(area 4). If the plate type you select has two drops per well, you can configure the zoom for both drops
1 and 2 through the
Advanced
option.
Selecting
Locate
instructs ROCK IMAGER to use the
Drop Location
algorithm to locate drops. Type
the desired Zoom value in the
Zoom
box. ROCK IMAGER will use this setting when finding a drop
location. The
Missed Zoom
is available only when you select Locate as drop Location. If ROCK IMAGER
failed locating a drop, it will use the
Missed Zoom
setting.
If you are using ROCK IMAGER with FORMULATRIX Advanced Contrast Imaging Technology (FACIT),
you can control the illumination pattern — such as top-left, top-right, bottom-left, darkfield, and
brightfield — to be projected onto a drop through the
Illumination
list. If you select
Locate
, you must
also select a method from the
Method
list. You can select
Standard
,
Fluorescence
or
Lipid Phase
as
a method. The available Method options may vary, depending on the imager type you purchased.
Standard Drop Locator
Standard Drop Locator
settings (available from the
Visible
tab) instruct ROCK IMAGER how to
locate drops before imaging. You can define
Min Length (µm)
,
Max Length (µm)
,
White Pixel Min
,
White Pixel Max
,
Open Iterations
, and
Max Aspect Ratio
.
●
Min Length (µm)
— Controls the minimum number of µm in a blob for that blob to count as
a drop.
●
Max Length (µm)
— Controls the maximum number of µm in a blob for that blob to count as
a drop.
●
White Pixel Min
— The minimum range of pixel intensities maps to white when converting
a sobel image to a binary image.
●
White Pixel Max
— The maximum range of pixel intensities maps to white when converting
a sobel image to a binary image.
●
Open Iterations
— Determines the number of iterations the system runs when opening the
image in preparation for drop location.
●
Max Aspect Ratio
— The maximum ratio between the largest and the smallest diameter of
a blob that represents the drop.
Fluorescence Drop Locator (FRAP only)
Fluorescence Drop Locator
settings (available from the
FRAP
tab) instruct ROCK IMAGER on how
to locate drops while using FRAP imaging. You can define values for
Gamma
,
Polarizer
,
Exposure
,
Gain
,
Condenser
, and
Brightness
. The values that you define for these fields will determine the
settings used while ROCK IMAGER is searching for the drop.
●
Gamma
— Determines the darkness of the light source. The higher the Gamma value, the
darker the image will be.
●
Polarizer
— Determines the polarized color applied to the image. You can type a value
between 0 and 360 into this field and see how the color changes on the live image.
ROCK IMAGER User's Guide | Chapter 12: Using the Plate Type Editor
Drop Location
Note:
If the Standard Drop Locator settings appear grayed, select
Fixed
under the
Drop
Location
.
