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Another useful application of the Auto-Alignment function is the comparison of
multiple traces at different stages of growth.
Manual Alignment/Deletion
This function enables manual alignment or deletion of each, individual trace on a
graph. When the “Manual Alignment” tab is selected, the graph will initially be
aligned at the value set in the Auto Alignment box. While the Auto Alignment
function is used to align all traces on a graph to the same point, Manual
Alignment facilitates the alignment of individual traces upon a single graph at
different points. Select each trace to be manipulated using the “Trace Selection”
pull-down box to the right of the slider. The selected trace can be pulled to the
left and right using the slider. Use the slider to realign all three traces on the
graph.
NOTE:
The advantage of the Manual Alignment slider over the Auto Alignment
function is that Manual Alignment facilitates alignment of individual traces one at
a time; whereas, Auto Alignment resets all traces simultaneously.
The Manual Alignment selection also facilitates deletion of selected traces. First,
select the trace to be deleted by using the “Trace Selection” pull-down box. Now,
simply click on the “Delete” button. The Delete function can be used to delete a
trace from any graph containing multiple traces - not just graphs created by
dragging. There are 2 circumstances under which traces from a graph CANNOT
be deleted. These are:
If the graph contains only a single trace.
If all the wells or sectors from a vessel are graphed simultaneously, then
the trace for the very first well or sector cannot be deleted. If all wells
from a microplate are graphed simultaneously, this will be the trace for
Well A1.
The above two deletion rules also hold true for the target graphs onto which plot
overlays are created.
Selecting an Individual Point on the Trace
Use the mouse to select an individual point on a trace. Hold the mouse over the
point, and the time and date of the scan represented by that point will be
displayed. Double left-click on a selected point, and it will open the Vessel View
window for that scan. The Vessel View window cannot be opened by double
clicking on the points in an Average Graph.
