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3.
Click the “Continue” Button. An un-previewed window will open.
4.
The Basic Analyzer phase confluence requires Users to select a “Training
Image Collection” (red circle Figure 16) to determine the parameters that
will differentiate between the
Background
and the
Cells
within the
image. The “Training Image Collection” should be the Image Collection
that you made containing 3-6 representative images of your cells of
interest (refer to 7.5.2.1).
5.
The images previewed are part of the Image Collection
(black circle
Figure 16) that you have selected at the top of the screen (Preview Image
Collection). This will likely be the same Image Collection used as the
“Training Image Collection”.
NOTE:
The Processing Definition can be tested on several Image
Collections within the editor, if desired.
6.
Click the “Preview” button.
NOTE
: The best way to begin setting up the Processing Definition is to
use the preset values already contained within the Processing Definition
Editor, i.e. do not change “Segmentation Adjustment”, “Cleanup”, or
“Filters” at this time.
Figure 16.
Establishing a New Processing Definition