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1.
Once a representative image is chosen, click the “Add to Image
Collection” link under Analysis Job Utilities in the top right corner.
2.
The “Add Current Image to Image Collection” window opens. Users can
create an image collection under four Analysis Job Types using the drop
down menu: Basic Analyzer (training for HD, fluorescent object testing),
Angiogenesis, NeuroTrack™, or ScratchWound.
3.
Use the radio buttons to add the image to an Existing collection or create
a New collection.
4.
Select the Required Image Channels that will be used to train (phase
analysis) or test (fluorescent object count, NeuroTrack, Angiogenesis, or
ScratchWound) the desired processing definitions.
5.
Once all fields are completed click the “OK” button. Each image
collection should be limited to 3-6 representative images.
NOTE:
Adding too many images will prolong the development of the
Processing Definition.
7.5.2.2
New Processing Definition:
A processing definition is a stored set of
parameters that completely specifies how to process acquired images.
Currently there are four types of processing definitions; 1) Basic Analyzer,
which includes confluence, phase, and fluorescent object analysis, 2) Scratch
Wound, 3) Angiogenesis, and 4) NeuroTrack
TM
. The Basic Analyzer is available
on all models of IncuCyte Zoom and others are available depending on the
configuration of your system. Outlined below are the steps to make a
processing definition using the Basic Analyzer to train the IncuCyte™ ZOOM
software for phase confluence.
1.
Once an Image Collection is saved start a New Processing Definition by
clicking on the “New Processing Definition” link within the Vessel View
(found just underneath the “Add to Image Collection” link).
2.
A new window will open (Add New Processing Definition) that contains a
drop down menu for Users to select the Image Collection that they
would like to use, in this case, to train (phase confluence) their
processing definition on. Users are also able to use the “Search” button
to locate an Image Collection.
NOTE
: The default image collection will be the most recently made
Image Collection.
