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9. Deviations from the Beer-Lambert Law may occur at high and low concentrations giving non-linear response
during sample concentration measurements. For all new methods a linear range should be defined by the
preparation of a calibration curve. The quantitation mode may be used to construct such a curve against
which sample results are automatically measured.
10. Cuvettes and sample holders must be filled to a minimum level which covers the light path. All Jenway
spectrophotometers have a beam height of 15mm.
11. The instrument must be calibrated to zero absorbance/100% transmittance prior to taking readings. In the
spectrum measurement mode a baseline scan must be performed before performing a sample scan.
