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Section 8 - Quantitation

The quantitation measurement mode enables sample concentrations to be calculated using a standard curve. In
this mode a number of standard solutions covering a range of known concentrations are measured at a set
wavelength. The absorbance or % transmittance of these solutions is plotted to create a standard curve. Once the
standard curve has been created a sample of unknown concentration can be measured and the concentration
calculated using the standard curve. Touch the Quantitation icon on the main menu to enter this measurement mode.

8.1

Method Set up

The parameters which can be entered in this measurement mode are the wavelength, number of replicates for the
calibration standards and concentration units of the calibration standards.

8.1.1

Selecting a Wavelength

To adjust the wavelength, touch

and use the keypad to enter the required wavelength. Touch

to apply the entered wavelength and return to the method set up. The wavelength selected needs to be the same
for the measurement of the standards as for the unknown sample.

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Содержание Jenway 7410

Страница 1: ...Spectrophotometer 7410 and 7415 Instruction Manual JEN0001 Version 1 0 ...

Страница 2: ...Guidelines 12 Section 4 Instrument Set up 14 4 1 Start up Screen 14 4 2 Navigation 14 4 3 Methods 16 4 4 Results 16 4 5 Settings 16 4 5 1 Instrument Status 16 4 5 1 1 Instrument Language 16 4 5 1 2 Setting the Time 17 4 5 1 3 Setting the Date 17 4 5 1 4 Setting the Time Zone 18 4 5 2 Measurement Settings 18 4 5 3 Network Connections 19 4 5 4 Storage 19 4 5 5 Service Settings 19 Section 5 Photometr...

Страница 3: ...ample Measurement 27 7 4 Data Analysis 28 7 4 1 Peaks and Valleys 28 7 4 2 Area Under Curve 29 7 4 2 1 Area Under Curve Baseline Mode 29 7 4 2 2 Area Under Curve Tangent Mode 30 Section 8 Quantitation 31 8 1 Method Set up 31 8 1 1 Selecting a Wavelength 31 8 1 2 Selecting Number of Replicates 32 8 1 3 Selecting Concentration Units 32 8 2 Measuring Calibration Standards 33 8 3 Standard Curve 34 8 4...

Страница 4: ...5 11 2 1 Loading Methods from Internal Memory 45 11 2 2 Loading Methods from USB Memory Stick 45 11 3 Deleting Methods 45 11 4 Saving Results 46 11 4 1 Saving Results to Internal Memory 46 11 4 2 Saving Results to CPLive 46 11 4 3 Saving Results to USB Memory Stick 46 11 5 Loading Results 46 11 5 1 Loading Results from Internal Memory 46 11 5 2 Loading Results from USB Memory Stick 46 11 6 Deletin...

Страница 5: ... 13 2 1 Tungsten Halogen Lamp Replacement 57 13 2 2 Xenon Lamp Module Replacement 58 13 3 Service Repairs and Support 58 13 4 Warranty 58 Section 14 Environmental Protection 58 14 1 Packaging Material 58 14 2 Waste Electrical and Electronic Equipment Directive WEEE 58 Section 15 Technical Specification 59 15 1 General Specification 59 15 2 Weights and Dimensions 60 Section 16 Troubleshooting 61 Se...

Страница 6: ...of the following safety advice SHOCK HAZARDS OR UNSAFE PRACTICES ARE DANGEROUS as they can cause severe personal injury fire or death DO NOT use combustible substances near hot objects DO NOT use the equipment in hazardous atmospheres DO NOT operate or handle any part of the equipment with wet hands or use on surfaces that may become flooded NEVER move the equipment while still connected to the po...

Страница 7: ...e for connecting to the power supply replace the plug with a suitable alternative THIS OPERATION SHOULD ONLY BE UNDERTAKEN BY A QUALIFIED ELECTRICIAN NOTE Refer to the equipment rating plate to ensure that the plug and fusing are suitable for the voltage and wattage stated The wires in the mains cable are as follows 230 V a c 120 V a c HOT LIVE BROWN BLACK HOT LIVE NEUTRAL BLUE WHITE NEUTRAL EARTH...

Страница 8: ...ot exceeding 10 of nominal Overvoltage category II IEC60364 4 443 Pollution degree 2 IEC664 Use with a minimum distance all round of 300mm 12in from walls or other items Place the equipment on a clean firm level surface which is free from drafts Avoid installation on a slippery surface or on a surface prone to vibration or on a surface prone to flooding Select the power lead and attach to the powe...

Страница 9: ...e 4 Removable panel for sipper accessory 5 2 x USB Type A ports 6 On Off power switch Rear View 7 Ethernet RJ 45 port 8 USB Type B port 9 Power inlet socket Underneath View 10 Lamp holder cover Refer to Section 13 2 for instructions on how to remove and replace the lamp 1 2 3 4 10 5 6 7 4 8 9 ...

Страница 10: ...r Lambert law states that A Ɛ l c A is the absorbance Ɛ is the molar absorption coefficient l mol 1cm 1 l is the path length cm c is the concentration mol I 1 This law shows that absorbance is linear to concentration but this is only true for low concentrations For absorbance levels above 3 the concentration starts to move away from the linear relationship Transmittance is the proportion of the li...

Страница 11: ...ing with 1200 lines per millimetre which separates the light into discrete wavelengths The diffracted spectrum of light then passes through a further slit and lens arrangement before passing through the sample in the sample chamber from left to right The light which is not absorbed by the sample is transmitted through a collecting lens and onto the signal detector The photo diode detector used is ...

Страница 12: ...nts are made f Ensure any sample containers used are compatible with the constituents of both the samples and standards they are to hold Plastic cuvettes are not compatible with organic solvents g All sample containers must be handled with care by the top bottom and non optical surfaces only Any finger marks evident must be removed by a suitable cleaning process h Flow through cuvettes must be sel...

Страница 13: ...titation mode may be used to construct such a curve against which sample results are automatically measured 10 Cuvettes and sample holders must be filled to a minimum level which covers the light path All Jenway spectrophotometers have a beam height of 15mm 11 The instrument must be calibrated to zero absorbance 100 transmittance prior to taking readings In the spectrum measurement mode a baseline...

Страница 14: ...wn below 4 2 Navigation The main menu is shown below These spectrophotometers are controlled solely through the touchscreen interface of the equipment and follow a basic Android user interface If the number of options available in a menu exceeds the number that can be displayed on the screen swipe to the left to view the other modes Use the keys on the navigation bar to move between screens ...

Страница 15: ...ent mode there is an overflow icon giving additional save and load method options Touching gives options to load a previously saved method touch to save the entered method parameters touch to upload to CPLive or touch to clear recently used method parameters Refer to CPLive instructions for more information When required to enter numbers a keypad will pop up Touch the required numbers and touch to...

Страница 16: ...11 for more information 4 5 Settings Touch to enable access to instrument status measurement settings network connections storage and service settings 4 5 1 Instrument Status Touch to view the status of the spectrophotometer check connections fitted accessories the serial number of the unit and the software version the instrument is using The language and date and time can also be set here 4 5 1 1...

Страница 17: ... each measurement mode and will be recorded against saved methods and results 4 5 1 3 Setting the Date To set the instrument date touch Scroll up or down to change the month viewed Touch the required date and touch to apply To set the year touch and scroll up or down and touch the required date and touch to apply Touching will return to the instrument settings screen without altering the date The ...

Страница 18: ...urement Settings Touch to select options for autosave results and the favourites panel Results can be saved automatically to the instrument s internal memory by sliding the button to the position To view the favourites panel on the home screen slide the button to the position If auto save is not selected then results need to be saved manually Refer to section 11 4 for more details ...

Страница 19: ... Storage Touch to view the amount of available storage on the internal memory of the spectrophotometer If a USB memory stick is inserted the amount of free space on the USB stick will also be shown 4 5 5 Service Settings Service settings is for service engineers only Touch to view the serial number and options to change serial number and to open windchime ...

Страница 20: ...asurement mode Touch the Photometrics icon on the main menu to enter this measurement mode 5 1 Method Set up This measurement mode is very simple and the only parameter which can be adjusted is the wavelength Once the required wavelength has been entered a calibration can be performed 5 1 1 Selecting a Wavelength To adjust the wavelength touch and use the keypad to enter the required wavelength To...

Страница 21: ...e to measure a sample before the instrument has been calibrated at the selected wavelength Once the calibration has been performed the icon becomes active and a sample can be measured Remove the cuvette containing the blank solution and place a cuvette containing the sample to be measured in the sample holder Close the instrument lid and touch the icon Once the measurement is completed the photome...

Страница 22: ...ters which can be entered in this measurement mode are wavelength factor or standard concentration and units of concentration Once all the required parameters have been entered a calibration can be performed 6 1 1 Selecting a Wavelength To adjust the wavelength touch and use the keypad to enter the required wavelength Touch to apply the entered wavelength and return to the method set up 6 1 2 Usin...

Страница 23: ...to the method set up To return to using a factor touch the icon and the use standard option will be disabled 6 1 4 Selecting Concentration Units The units of concentration can be selected from several options Touch to select from the menu Touch the circle adjacent to the required unit of concentration The selected unit will be displayed against the final concentration result 6 2 Calibration The ca...

Страница 24: ...cuvette containing the blank solution into the sample chamber and close the instrument lid Touch the icon This sets the instrument to and If a standard concentration has been entered the icon will become active Insert a cuvette containing the known standard solution into the sample chamber and close the instrument lid Touch the icon and the instrument will measure the absorbance of the standard sa...

Страница 25: ...red in the sample chamber Close the instrument lid and touch the icon Once the measurement is complete the concentration absorbance and transmittance values are displayed 6 3 2 Measuring a Sample After Calibrating to a Standard Remove the cuvette containing the standard solution and place a cuvette containing the sample to be measured in the sample chamber Close the instrument lid and touch the ic...

Страница 26: ...The parameters which can be entered in this measurement mode are start and end wavelength scan interval and measurement mode Once all the required parameters have been entered a calibration can be performed 7 1 1 Setting Start and End Wavelengths Model 7410 can perform measurements from 320nm to 1000nm model 7415 can perform measurements from 198 to 1000nm To adjust the start wavelength touch and ...

Страница 27: ... The calibration must be performed across the same wavelength range as the sample will be measured across Insert a cuvette containing the blank solution into the sample chamber and close the instrument lid Touch the icon to initiate the baseline scan This sets the instrument to zero absorbance and 100 transmittance across the wavelength range and scan interval If the wavelength range or scan inter...

Страница 28: ... and Valleys To view the peaks and valleys touch The peaks and valleys table displays all the detected peaks and valleys above the selected threshold value Touch and slide to change the threshold level The graph can be zoomed in and out by pinching two fingers on the screen To add a point to the list touch the spectrum scan to open a sliding cursor Slide the cursor across the scan to the required ...

Страница 29: ... tangent touch or to select the required measurement mode Repeat touches will cycle between the two options 7 4 2 1 Area Under Curve Baseline Mode Baseline will calculate the area under the curve between the two sliding cursors Slide the cursors to the select the area required You can also use the arrows to move the selected area ...

Страница 30: ... Mode Tangent will calculate the area under the curve from the point where each of the two sliding cursors crosses the spectrum scan Slide the cursors to the select the area required You can also use the arrows to move the selected area ...

Страница 31: ...d and the concentration calculated using the standard curve Touch the Quantitation icon on the main menu to enter this measurement mode 8 1 Method Set up The parameters which can be entered in this measurement mode are the wavelength number of replicates for the calibration standards and concentration units of the calibration standards 8 1 1 Selecting a Wavelength To adjust the wavelength touch an...

Страница 32: ...e selected number of replicates If individual sample replicates are being used do not select the automatic replicates option 8 1 3 Selecting Concentration Units The units of concentration can be selected from several options Touch to select from the menu Touch the circle adjacent to the required unit of concentration The selected unit will be displayed against the final concentration result Once t...

Страница 33: ...nk into the sample chamber and close the lid Touch to perform a zero absorbance 100 transmittance Once the blank is complete remove the blank solution from the sample chamber and place the 1st known standard solution into the sample chamber and close the instrument lid Touch and the instrument will measure the absorbance of the standard sample If there are 2 or more replicates you will need to tou...

Страница 34: ...e asked to measure replicate 1 followed by the remaining replicates Touch to save the absorbance results for the standard Repeat the above process for the number of standards required to create the calibration curve A calibration curve can be set up in advance and concentrations saved for future use without measuring the absorbance values When the quantitation assay is next performed each standard...

Страница 35: ...nd correlation coefficient r Once all the standards have been measured touch and then the unknown samples can be measured 8 4 Sample Measurement Place a cuvette containing the sample to be measured in the sample holder Close the instrument lid and touch the Once the measurement is completed the concentration and photometric results will be shown on the screen Subsequent samples can be measured in ...

Страница 36: ...easurements analysis of all or part of the experiment can be performed 9 1 Method Set up The parameters which can be entered in this measurement mode are wavelength run time measurement time interval lag time absorbance or transmittance measurement mode and the concentration parameters Once all the required parameters have been entered a calibration can be performed 9 1 1 Selecting a Wavelength Th...

Страница 37: ...it before starting the kinetics measurements after the Sample icon has been touched To set the lag time touch Scroll up or down beneath Hours Minutes Seconds to select the required time and touch to apply Touch to exit the lag time set up without saving the changes 9 1 5 Selecting Absorbance or Transmittance The default operating mode is absorbance To change this between absorbance or transmittanc...

Страница 38: ...ument to zero absorbance and 100 transmittance 9 3 Sample Measurement It is not possible to measure a sample before the instrument has been calibrated at the selected wavelength Once the calibration has been performed the Sample icon becomes active and a sample can be measured Remove the cuvette containing the blank solution and place a cuvette containing the sample to be measured in the sample ho...

Страница 39: ...age will appear asking for confirmation to stop the kinetics run Touch to stop the run touch to carry on with the kinetic run Once the measurement time is complete the full kinetics run will be shown on the screen To run the kinetics experiment again using the same method parameters touch Subsequent samples can be measured in the same way If the wavelength is adjusted between sample measurements t...

Страница 40: ...e over time can be viewed for the entire kinetics run or for selected parts of the kinetics run Touch on the graph and slide to the required start or end point in the kinetics run The rate of change will automatically update If the end point is moved this will automatically update the end point concentration Alternatively touching will move the start or end point lines to the required time Touch t...

Страница 41: ...ers which can be entered in this measurement mode are wavelength type of equation factor measurement mode and units of concentration Once all the required parameters have been entered a calibration can be performed 10 1 1 Selecting a Wavelength The wavelength value can be adjusted by touching and then using the keypad to enter the required wavelength Touch to apply the entered wavelength and retur...

Страница 42: ...ype of equation can be selected from several options Touch and select the required equation from the menu If no equation is selected then the factor and units options will be disabled Touch to apply the equation parameters or touch to return to the method set up without saving any changes ...

Страница 43: ...en the units of concentration will also need to be selected The units of concentration can be selected from several options Touch to select from the menu Touch the circle adjacent to the required unit of concentration The selected unit will be displayed against the final concentration result Touch to apply the concentration units or touch to return to the method set up without saving any changes 1...

Страница 44: ...ibrated at the selected wavelengths Once the calibration has been performed the icon becomes active and a sample can be measured Remove the cuvette containing the blank solution and place a cuvette containing the sample to be measured in the sample holder Close the instrument lid and touch the icon Once the measurement is completed the result will be shown on the screen Touch to measure subsequent...

Страница 45: ...rameters 11 1 3 Saving Methods to USB Memory Stick You can save your methods to USB memory stick via the home or method set up screen On the home screen touch Touch you can then select each method individually by touching by the side of each method or touch to select all the methods Touch the overflow icon and then touch On the method set up screen touch and then touch to save the entered method p...

Страница 46: ... select all the methods Touch the overflow icon and then touch 11 5 Loading Results 11 5 1 Loading Results from Internal Memory On the home screen touch Touch the required result from the list to view information 11 5 2 Loading Results from USB Memory Stick This option is currently unavailable 11 6 Deleting Results On the home screen touch Touch you can then select each result individually by touc...

Страница 47: ...ccessories ensure that the equipment is cool and disconnect from the power supply There are two types of accessories which can be fitted in the sample chamber passive non powered or active powered accessories The range of passive accessories includes 10 x 10mm single cuvette holders adjustable path length 10 to 100 mm cuvette holders test tube holders and micro cuvette holders The active accessori...

Страница 48: ...w to undo the passive accessory Lift out the passive accessory Unscrew the screws 1 to 4 and lift out the metal base panel This will expose the bottom of the sample chamber and the power supply connection needed to operate the active accessories 1 2 3 4 ...

Страница 49: ...4 Take the 8 cell carousel and place on top of the motor taking care to align the three ball bearings with the grooves on the motor shaft Gently push the carousel down onto the motor shaft until it is located into place Gently rotate the carousel until there is some resistance The carousel is now in the correct position If the fitting is too tight use a small screw driver to loosen the ball bearin...

Страница 50: ... mode Select the measurement mode you require photometrics has been used as an example only the automatic 8 cell turret will automatically move to position 0 The current cell position will be displayed Set up the required measurement parameters Insert the cuvette containing the blank solution into turret position 0 followed by the cuvettes containing the samples into turret positions 1 to 7 and cl...

Страница 51: ...can also change the cell position by touching and then selecting the required position Touch to return to the method set up screen and touch to measure the sample Repeat the process until all the samples have been measured Once the measurement is complete the result will be shown At this point you can either or ...

Страница 52: ...Insert the cuvette containing the blank solution into turret position 0 followed by the cuvettes containing the samples into turret positions 1 to 7 and close the instrument lid Touch and then touch twice at position 1 This will activate the sample icon Touch once at each of the positions you have inserted samples into This will activate the sample icon Touch to apply changes and return to method ...

Страница 53: ...nce and 100 transmittance Once the calibration has been performed the turret will automatically measure each sample Once all of the samples have been measured the results will be displayed Touch the corresponding cell position to view the results At this point you will be able to or ...

Страница 54: ...e plastic visible wavelength 10x10 cuvettes 060 229 Pack of 500 disposable plastic visible wavelength 10x10 cuvettes 060 230 Pack of 100 disposable plastic UV wavelength 10x10 cuvettes 037 702 Paper roll for printer 740 093 24V 65W power supply unit HH179 S UK mains lead HH180 S European mains lead FCABLEUS USA mains lead Please contact your local sales specialist or email cpspares coleparmer to e...

Страница 55: ... have different filament configurations or be wavelength restricted for domestic or commercial use and will give errors if used WARNING This product does not contain bio seals as per EN 61010 1 2010 and cannot provide any level of containment in case of a spill or release of toxic radioactive or pathogenic micro organisms thus these materials are not recommended to be used in this product NOTE Do ...

Страница 56: ...iring service the item s should be returned to the manufacturer for repair accompanied by a decontamination certificate Copies of the Certificate are available from the Distributor Manufacturer At the end of its service life the product must be accompanied by a Decontamination Certificate ...

Страница 57: ...d slightly to allow it to be removed V Carefully remove the replacement lamp from the packaging Ensure that the glass portion of the lamp is not touched as finger marks will damage the lamp resulting in a reduced performance If accidental damage occurs the surface of the lamp may be cleaned using propan 2 ol VI Insert the new lamp assembly into the same position as the one just removed This may ne...

Страница 58: ...0 1785 810433 13 4 Warranty Cole Parmer Ltd warrants this instrument to be free from defects in material and workmanship when used under normal laboratory conditions for a period of 3 years In the event of a justified claim Cole Parmer will replace any defective component or replace the unit free of charge This warranty does NOT apply if damage is caused by fire accident misuse neglect incorrect a...

Страница 59: ...0 standards Curve fit algorithms Linear through zero linear quadratic through zero and quadratic Kinetics Measurement Time 2 to 9999 seconds Calibration Blank with a single standard or factor Display Graphical Analysis Concentration rate of change initial and final absorbance T Spectrum Range 320 to 1000nm 198 to 1000nm Scan interval Selectable 1 2 5 or 10nm Analysis Absorbance or transmittance an...

Страница 60: ...60 15 2 Weights and Dimensions Weight 9 0kg 280mm 156 mm 500mm 156 mm ...

Страница 61: ...ed Contact Cole Parmer support and quote error code 105 A hardware problem has been detected Contact Cole Parmer support and quote error code 106 A hardware problem has been detected Contact Cole Parmer support and quote error code 107 A potential hardware problem has been detected If there s currently a sample in the instrument remove it and try again If there s no sample in the instrument contac...

Страница 62: ...ing airflow and turn the unit off to allow it to cool down If this continues to occur contact Cole Parmer support and quote error message 114 The blank standard sample failed because a potential hardware fault detected with the fan The fan has dropped below its normal operating speed Check the air vents for any obstructions which may be interrupting airflow and then turn the instrument off and on ...

Страница 63: ...w Blank Blank complete Apply Select files Cancel Unused turret position Delete Select all Deselect favourite Favourite Load method Save as method Measurement Settings Clear recent Information Overflow Accessory attached Printer Take reading Save Search Settings Spectrum peak Spectrum valley Standard complete Stop Results Turret Export to USB Hide favourites panel Network connections Service settin...

Страница 64: ...ation 8 Installation Conditions 8 Installing Automatic 8 Cell Turret 49 Installing the Accessories 47 Instrument Language 16 Instrument Set up 14 Instrument Status 16 Introduction 6 K Kinetics 36 Calibration 38 Data Analysis 40 End Point Concentration 37 Method Set up 36 Sample Measurement 38 Selecting Absorbance or Transmittance 37 Selecting a Wavelength 36 Setting Lag Time 37 Setting the Kinetic...

Страница 65: ...46 Saving Results to USB Memory Stick 46 Service Repairs and Support 58 Service Settings 19 Settings 16 Setting the Date 17 Setting the Time 17 Setting the Time Zone 18 Spare Parts 54 Spectroscopy Measurement 11 Spectrum 26 Area Under Curve 29 Area Under Curve Baseline Mode 29 Area Under Curve Tangent Mode 30 Calibration 27 Data Analysis 28 Method Set up 26 Peaks and Valleys 28 Sample Measurement ...

Страница 66: ...66 ...

Страница 67: ... Cole Parmer Representative Beacon Road Stone Staffordshire ST15 0SA United Kingdom Object of Declaration Scanning Spectrophotometer Range 74 Series reference the attached list of catalogue numbers The object of the declaration described above is in conformity with the relevant Union Harmonisation Legislation Additional Information Place of Issue Date of Issue Authorised Representative Title Signa...

Страница 68: ...Cole Parmer Ltd Beacon Road Stone Staffordshire ST15 0SA United Kingdom Tel 44 0 1785 812121 Email cpinfo coleparmer com Web www jenway com ...

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