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8.2
Measuring Calibration Standards
The measured standards are used to create a calibration curve. If there is only one standard available the
concentration measurement mode should be used. Touch
to add the first standard.
Touch
to use keypad to enter the concentration value required for that standard. Touch
to apply.
Before the 1st calibration standard is measured a blank sample must be measured first. Insert a cuvette containing
the blank into the sample chamber and close the lid. Touch
to perform a zero absorbance, 100%
transmittance. Once the blank is complete remove the blank solution from the sample chamber and place the 1st
known standard solution into the sample chamber and close the instrument lid. Touch
and the instrument
will measure the absorbance of the standard sample. If there are 2 or more replicates you will need to touch
.
Once replicate 1 has been measured, replace it with the next replicate and repeat the process until all replicates
have been measured. If using automatic replicate measurement, the same standard will be read for the selected
number of times.