Biocontrol CL-10 Plus, Руководство пользователя

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CL-10 Plus – User Manual
65 / 75 ECPUS vers 0.2 eng
6.2. Fluidic Section.
6.2.1 The Mixer does not fill-in or empty.
•
Check that there are no broken, chocked or obstructed peristaltic tubes
•
Check that the connections to the pumps are not inverted (electrode 1 connected to
pump 1, electrode 2 connected to pump 2).
•
Place a bottle of distilled water in buffer position and manually turn the buffer pump
(pump 4) clockwise; check that the water is correctly loaded and reaches the mixer.
If any defect is found replace the pump tube and inspect the fluidic line.
•
When the mixing chamber is full, manually rotate the pump of the Electrode n.1
(pump 1) clockwise and empty the Mixer.
•
If the mixing chamber does not empty, check if tubes are broken or obstructed and if
there is any leakage.
•
Fill in the mixing chamber and repeat the last step with the pump of Electrode n. 2
(pump 2).
•
Turning pump n.4, fill the mixing chamber up to the top. Manually rotating the Level
pump n.3 clockwise, check that it levels.
•
Carry out a measurement ( Sample command) and check that the magnetic stirrer inside
the mixing chamber start rotating at the beginning of the measurement for approx. 4
seconds. If you cannot hear it, insert a pipette tip in the chamber, you should feel the
collision with the bar.
•
If not, ask for technical service.
6.2.2 Liquid spills out from the mixing chamber.
If some liquid spills out from the mixing chamber during the cleaning, it is possible that the
fluidics have an obstruction.
In this case follow the procedure to ‘release’ electrodes channels from sediments,
obstructions, etc:
1. Place the wash solution (Polif kit ) in place of the working buffer,
2. Run 2
Clean cycles using clean icon or F3 function key,
3. Unplug the pump 1 tubing from its place around the rotor,
4. Take the connection between the pump tubing and the waste off,
5. Connect a 10mL syringe to the loose end of the pump tubing and pull/push the piston
for few seconds.
ATTENTION: during this phase, pushing the piston, some liquid could flow out the top of the mixing chamber.
Therefore, it is advisable, with the hand, to keep a tissue on the black stopper of the chamber and push the
piston gently.
6. Run 2 Clean cycles using clean icon or F3 function key
7. Repeat the same procedure with electrode 2 (pump 2).
8. Run 4
Clean cycles using clean icon or F3 function key and leave the instrument in
standby until the following working session.