Biocontrol CL-10 Plus, Руководство пользователя

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CL-10 Plus – User Manual
59 / 75 ECPUS vers 0.2 eng
5.4. Enzyme Contamination in the reference electrode
When Check problem description solution
Every
working
session Enzyme
Contamination in
the reference
electrode If there is a D1 drift, like in
Figure
2,
an electrode contamination is
possible (in case of urea, the
correct description of the problem
is
urease contamination
).
The problem affects the method
performances mainly at high
concentrations (Figure 2).
The result is a set of unreliable
data, low repeatability and frequent
failure of calibration/controls
checks.
1.
Using a Pasteur pipette, empty,
reaching the bottom of mixing
chamber, it from the top.
2.
Remove the enzyme tubing from
the top pf the mixing chamber
3.
Fill in the chamber with 1 mL of
HCL 0,1M and run a
measurement (GO or F5)
4.
Inject 1 mL milk (any kind) and
run 3
Clean cycles (F3)
5.
Reconnect the enzyme tube to the
top of the mixing chamber
6.
Proceed to the working session,
according to the package insert of
the kit.
Every
working
session Kind of measure
‘end point’
see § 3.3.2 The curve, at the end of the
reaction, has a final slope=0.
If not, obtained results are neither
trustful nor repeatable.
The reason is:
1) old peristaltic pump tubing’s,
with no optimal sealing properties
or
2) a blockage in the system. Replace peristaltic pump tubing’s.
Check if the stirrer bar, inside the
mixing chamber, is rotating (insert the
Microman M25 tip in the chamber at
the end of the measurement and ‘feel’
the bar movements.
The enzyme could be damaged or
expired; replace with a new vial from
the same kit or another kit of the same
Lot n°.