Applied Biosystems GeneAmp PCR System 9700, Руководство пользователя

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Converting Hold Times 6-3
Guidelines for Converting Hold Times
Introduction
This section describes guidelines on how to convert hold times for the DNA Thermal
Cycler or the DNA Thermal Cycler 480 to hold times for the GeneAmp PCR
System 9700 when using the 9600 mode.
What to Base the
New Hold Times On
The following table lists what to base the new hold time on if you are using an up ramp
or down ramp.
Setting Up Ramps
The following table lists the process of setting the up ramp temperature.
Table of Conversions
The following table lists the times for converting up ramp hold times greater than one
minute from the DNA Thermal Cycler and the DNA Thermal Cycler 480 to the
GeneAmp PCR System 9700.
If you are using... Then base the new hold time... For more information see...
an up ramp on the change in the temperature
required to reach the next target
temperature. Table 6-1 on page 6-3.
a down ramp on the starting temperature of the
ramp and the change in
temperature required to reach the
next temperature. Table 6-2 on page 6-5.
Step Action
1 Determine the change in temperature required to reach the next target temperature
and round this value off to the closest value found in Table 6-1.
2 Subtract the number of seconds indicated from the hold time used for the DNA
Thermal Cycler or the DNA Thermal Cycler 480.
The result is the hold time to use for the GeneAmp PCR System 9700.
Note The typical hold time is 10 to 15 seconds for denaturation.
Table 6-1 Converting up ramp hold times
∆
Temp (
°
C)
Seconds to subtract from DNA Thermal Cycler or
DNA Thermal Cycler 480 hold times (>1 min.)
10
°
38 sec.
15
°
45 sec.
20
°
49 sec.
30
°
54 sec.
40
°
55 sec,
50
°
57 sec.
60
°
57 sec.