Applied Biosystems GeneAmp PCR System 9700, Руководство пользователя

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6-2 Converting Hold Times
About Setting Hold Times
Introduction
The hold times specified for the GeneAmp PCR System 9700 are shorter than those
used for the Step-Cycle or ThermoCycle files on the DNA Thermal Cycler and the
DNA Thermal Cycler 480. This is because the DNA Thermal Cycler and the DNA
Thermal Cycler 480 starts counting the hold time when the block reaches a
temperature one degree before the target temperature, while the GeneAmp PCR
System 9700 starts counting the hold time when the samples reach a temperature
one degree before the target temperature.
Hold Time
Differences
As shown in the figures below, since the block reaches the target temperature before
the sample, the programmed hold time on the DNA Thermal Cycler and the DNA
Thermal Cycler 480 must include enough time for the samples to reach the target
temperature. A hold time of one minute or greater is required on the DNA Thermal
Cycler and the DNA Thermal Cycler 480 for samples to reach the target temperature.
On the GeneAmp PCR System 9700, hold times of less than one minute are generally
used.
DNA Thermal Cycler and DNA Thermal Cycler 480
Hold Time
Block
Sample
1 minute Time
Temp
(˚C)
94
93
Clock starts
when block
reaches target
temperature
GeneAmp PCR System 9700
Hold Time
Block
Sample
Time
Temp
(˚C)
94
93
Clock starts
when sample
reaches target
temperature