Applied Biosystems 2720, Руководство пользователя

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57 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 5 Temperature non-uniformity test
Running the test
The temperature non-uniformity test uses the 0.02-mL probe assembly to test the
temperature uniformity of six different wells in each sample block.
Note:
If necessary, press F5 (Cancel) to exit the test.
To run the temperature non-uniformity test:
1. Complete the procedures in “Setting up the 0.02-mL probe assembly” on
page 55 .
2. Press F1 (Run) to start the temperature non-uniformity test. The TNU
Performance screen opens with the setpoint value displayed.
Note:
The sample blocks must be within
±
1.0 °C of the setpoint. In addition,
the cover must be within
±
1 °C of 105 °C. It may take several minutes for the
GeneAmp
®
9700 to ramp up.
3. The TNU Performance screen counts down the time until the setpoint is stable.
When the “Stabilizing at setpoint” value decreases to 0, read the digital
thermometer. Refer to the instructions in your Temperature Verification Kit for
details on operating the digital thermometer, Model 4500.
4. Using the numeric keys, enter the value that is displayed on the digital
thermometer in the “Enter actual block temperature” field.
Note:
Round off the four-digit value on the digital thermometer to three digits
before you enter it into the Calibration Verification screen.
Note:
Record this value on the Temperature Non-Uniformity Test Data Sheet
( page 61 ) to keep a permanent record of the test.
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
Setpoint is 94°C
Sample must be within 1.0°C of setpoint
F1 F3 F4 F5 F2
Cancel
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
Stabilizing at setpoint... x:xx
F1 F3 F4 F5 F2
Cancel
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
F1 F3 F4 F5 F2
Cancel
Enter actual block temperature 00.0