Applied Biosystems 2720, Руководство пользователя

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55 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 5 Temperature non-uniformity test
Temperature non-uniformity test
Use this test to verify the temperature uniformity of the GeneAmp
®
PCR System
9700, Dual 384-Well Sample Block Module. The temperature non-uniformity test
consists of the following procedures, which you must perform in sequence:
Setting up the 0.02-mL probe assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Running the test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Evaluating the results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Required
equipment
This test requires the Dual 384-Well Sample Block Module Temperature Verification
Kit
(PN 4308354), which includes:
• Light mineral oil
• Left and right 384-well temperature verification plates
• 0.02-mL probe assembly
• Digital thermometer Model 4500 with a 9V battery installed
Setting up the
0.02-mL probe
assembly
1. If the heated cover is in the forward position, lift the lever, then slide the heated
cover back.
2. Place both 384-well temperature verification plates on the sample blocks.
IMPORTANT! The temperature verification plates must be installed in both
sample blocks throughout all temperature verification testing.
The plate labeled L1 to L6 is for the left sample block ( Figure 12 ). The plate
labeled R1 to R6 is for the right sample block ( Figure 13 ). Position the plates so
the angled corners face away from you.
Note:
The plates help you locate the probes within the sample blocks.
3. Use the stick-end of a cotton swab to coat wells L1 to L6 and R1 to R6 with
mineral oil; the wells are too small for the swab-end.
4. Place the 0.02-mL probe assembly into well L1.
Note:
As the test progresses, you move the 0.02-mL probe assembly to each of
the test wells (L1 to L6 and R1 to R6).
Figure 12 Left temperature
verification plate
Figure 13 Right temperature
verification plate