Applied Biosystems 2720, Руководство пользователя

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4 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 1 About this manual
Temperature
verification tests
You use the Temperature Verification System to perform the following tests on your
GeneAmp
®
PCR Systems, Veriti
®
Thermal Cycler, and 2720 Thermal Cycler:
• Temperature calibration verification test – Checks the temperature of the
sample block against specifications for temperature accuracy
• Temperature non-uniformity test – Checks the uniformity of sample block
temperature from well to well
You can perform these tests as frequently as necessary, depending on the type of
work your laboratory performs. For example, a laboratory that does forensic typing
may need to validate the instrument before or after running samples to have the test
results considered valid in a court case. Another laboratory may want to validate a
protocol.
Typically, the temperature calibration verification test should be performed at least
once a year, and the temperature non-uniformity test should be run at the same time
and frequency.
Running these tests verifies that the thermal cycler is operating to Applied
Biosystems operating specifications.
Kit parts The kit is supplied with a storage case ( Figure 2 ) that includes:
• Model 4500 digital thermometer with a 9V battery installed
• Probe assembly
• Cotton swabs
• Light mineral oil
• Temperature verification frames
Figure 2 Temperature Verification Kit
Figures 1 and 2 show the major components of the kit.
Note:
If any part is damaged or missing, contact the shipping carrier and Applied
Biosystems immediately. See “Damages, claims, and returns” on page 134 .
Recalibration
Applied Biosystems recommends that your Temperature Verification System be
recalibrated once a year. For instructions on having your system recalibrated by the
manufacturer, refer to
Appendix A, “Recalibration.”