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Freezing Point/Melting Point

: The temperature at which the liquid

and solid phases of a substance will remain together in equilibrium.

Freezing-Point Depression

: When a solute is added to a solvent, the

freezing point of the solvent is lowered. In aqueous solutions, one

mOsm of solute per kilogram of water depresses the freezing point by

1.858 millidegrees Celsius (m°C).

Supercooling

: The tendency of a substance to remain in the liquid state

when cooled below its freezing point.

Crystallization Temperature

: Aqueous solutions can be induced to

freeze (i.e., crystallize) most reliably when supercooled. When super-

cooled, agitating the solution (freeze pulse) induces crystal formation.

The crystallization temperature is the temperature at which crystalliza-

tion is induced. During crystallization, the heat of fusion raises the tem-

perature of the sample to an ice/water freezing-point plateau.

Heat of Fusion

: The heat released when the mobile molecules of a liq-

uid are frozen into rigid ice crystals.

Freezing-Point Plateau

: The constant temperature maintained during

the time that ice and liquid exist in isothermal equilibrium after crystal-

lization occurs.

xviii

The Advanced

Osmometer Model 3900 User’s Guide

Содержание 3900

Страница 1: ...The Advanced Osmometer Model 3900 User s Guide 3905 Rev20 061213 ...

Страница 2: ...nstruments Inc Advanced Instruments has reviewed this guide thoroughly All material contained within is believed reliable but the accuracy and completeness are not guaranteed or warranted and are not intended to be representations or warranties concerning the product described Hot Line Service If you have any questions or problems regarding the proper operation of your instrument please contact ou...

Страница 3: ...i Chapter 1 Installation Setup 1 Step 1 Find a location for your instrument 1 Step 2 Unpack your instrument 2 Step 3 Setup and installation 3 Step 4 Load printer paper 7 Step 5 Obtain additional items 9 Step 6 Power up your instrument 9 Step 7 Install heat transfer fluid 10 Step 8 Run self diagnostic test 12 Step 9 Proceed to instrument operation 12 Chapter 2 Instrument Operation 13 Hazardous mate...

Страница 4: ... 5 Troubleshooting Service 51 Service maintenance cautions 51 Obtaining service 53 Routine maintenance 55 Shutdown and storage 56 Troubleshooting 56 Test 57 Adjustment and replacement of parts and assemblies 67 Appendices 79 Appendix A Troubleshooting Table 79 Appendix B Product Specifications 91 Appendix C Regulatory Notices 93 Appendix D Warranty and Warranty Duties 97 Appendix E Supplemental RS...

Страница 5: ...ESCRIPTION Calibration Cassette Disposable Sample Tubes 0 2 to 0 3 mL one pkg of 500 Sample Tube Cassettes 1 5 Sample Tube Cassettes 6 10 Sample Tube Cassettes 11 15 Sample Tube Rack Heat Transfer Fluid one 160 mL bottle Cooling Reservoir Fill Funnel Thermal Printer Paper one pkg of 5 Evaporation Cover Fluid Fill Plug vented PART NO 4C3040 3LA825 4C3050 4C3060 4C3070 3LA846 3DA811 4C3308 FLA835 39...

Страница 6: ...ith three 10 mL ampules of Probe Bin Setting Fluid Probe Stir Alignment Kit Mandrel Clapper Replacement Assembly Yoke Stir Freeze Coil Serial Port Computer Cable with DB9S 3 Meter Shielded RS 232 6 Meter Shielded RS 232 9 Meter Shielded RS 232 3900 User s Guide 3900 Service Manual Bar Code Reader with Stand and Instructions one kit PART NO FL0408 FL0425 4C3840 3D2340 3D3185 3C2243 4C3700 3LA700 39...

Страница 7: ... 100 500 900 1500 and 2000 mOsm kg ten 2 ea 5 mL ampules 100 mOsm kg Calibration Standard ten 5 mL ampules 500 mOsm kg Calibration Standard ten 5 mL ampules 900 mOsm kg Calibration Standard ten 5 mL ampules 1500 mOsm kg Calibration Standard ten 5 mL ampules 3000 mOsm kg Calibration Standard ten 5 mL ampules 100 mOsm kg Calibration Standard one 110 mL bottle 500 mOsm kg Calibration Standard one 110...

Страница 8: ...are used with the instrument Use of consumables from manufacturers other than Advanced Instruments is not recommended and may adversely affect system calibration performance operation and accuracy of test results For information on obtaining these standards and controls contact Advanced Instruments or an authorized representative The Advanced Osmometer Model 3900 User s Guide ...

Страница 9: ...itute for electrical training Symbol conventions The exclamation point within an equilateral triangle is intended to alert the user to the presence of important operating and maintenance servicing instructions in the literature accompanying this product The lightning flash with arrowhead symbol within an equilateral triangle is intended to alert the user to the presence of uninsulated dangerous vo...

Страница 10: ... must be approved for the product s listed operating voltage and be rated at least 20 greater than the ampere ratings marked on the product s electrical ratings label The cord end that connects to the product must have an IEC 60320 connector Plug the product into an approved grounded electrical outlet Do not disable the power cord s grounding plug If an extension cord or power strip is used make s...

Страница 11: ...erm temperature variations or locations that exceed the speci fied operating environment temperatures Never use this product in a wet area To avoid injury or fire hazard do not operate this product in an explosive atmosphere Do not install or use the product on an unstable non level work sur face Do not operate this product with the covers removed or unsecured xi Safe Use ...

Страница 12: ...xii The Advanced Osmometer Model 3900 User s Guide Notes ...

Страница 13: ... and pharmaceutical research and manufacturing Food and beverage manufacturing Environmental research and monitoring Academic research Industrial applications When used by a trained operator in clinical applications the osmometer provides results that assist in establishing the proper diagnoses and treatments for patients with disorders involving water and electrolyte imbalances Osmometers will te...

Страница 14: ...mined with great precision The freezing point of pure H2O is precisely 0 010 C One mole of a non dissociating solute such as glucose where the solute does not dis sociate into ionic species but remains intact when dissolved in 1 kilo gram kg of water will depress the freezing point by 1 858 C This change is known as the freezing point depression constant for water The freezing point depression als...

Страница 15: ...ity most osmometry applications regularly use osmolality expressed as mOsm kg H2O as the common unit of concentration rather than applying further conver sion factors Instrumentation Advanced Osmometers are devices for the determination of the concen tration of solutions by means of freezing point measurement Advanced Osmometers utilize high precision thermistors to sense the sample temperature to...

Страница 16: ...s monitor the sample temperature and control the thermoelectric cooling element Microprocessor control and auto mated operation minimize imprecision due to operator technique The following Standard Freezing Curve illustrates the temperature of a sample as it progresses through the freezing cycle and shows the action of the instrument at each stage of the cycle Definitions Solution A homogeneous mi...

Страница 17: ... H2O One milliosmol mOsm is 10 3 osmols Osmolality is defined as where ø osmotic coefficient which accounts for the degree of mole cular dissociation n number of particles into which a molecule can dissociate C molal concentration of the solution Molarity Moles of solute per liter of solution Osmolarity Osmols of solute particles per liter of solution Although molarity and osmolarity may be common...

Страница 18: ...ture Aqueous solutions can be induced to freeze i e crystallize most reliably when supercooled When super cooled agitating the solution freeze pulse induces crystal formation The crystallization temperature is the temperature at which crystalliza tion is induced During crystallization the heat of fusion raises the tem perature of the sample to an ice water freezing point plateau Heat of Fusion The...

Страница 19: ...51 55 55 cm Be sure to keep your workplace free of debris especially around the back and sides of the instrument where proper ventilation is need ed NOTE Bright light shining directly into the cassette deck area can interfere with the operation of the instru ment Electric outlet availability Your instrument will need to operate within five feet of a properly grounded three prong electrical outlet ...

Страница 20: ...r accessories and supplies and inspect them for shipping damage Use the enclosed packing list to verify that all items have been received b Save your osmometer s shipping boxes and packaging material in case future transport of the instrument becomes necessary c If any item on the packing list appears to be missing from your ship ment please search carefully through and under all packing materi al...

Страница 21: ...ample Tube Cassettes set of 1 5 Calibration 1 pack 3LA011 100 mOsm kg Standard 1 pack 3LA151 1500 mOsm kg Standard 1 pack 3LA201 2000 mOsm kg Standard 1 pack 3LA301 3000 mOsm kg Standard included with extended range option 1 pack 3LA029 Clinitrol 290 Reference Solution 2 Rolls of Thermal Printer Paper reorder pack of 5 as FLA835 1 FL0425 Printer Maintenance Kit with Instructions 1 4C3448 Fluid Fil...

Страница 22: ...ion The following additional unpacking and installation procedures should be accomplished before power is applied to the instrument 1 Open the magnetically held drop down cassette deck cover see fig ure 2 by pulling its frame forward and down 2 Remove the two screws and top half of the operating head shipping 4 The Advanced Osmometer Model 3900 User s Guide Figure 2 Major Parts and Controls Displa...

Страница 23: ...wo screws and top half of fixture before powering instrument B Freezing chamber must be plugged during shipment Remove freezing chamber plug before powering instrument C A dry probe wiper must be used to protect probe during shipment Lift operating head and remove protective material from probe and stir freeze wire manually rotate operating head forward gently lower probe between decks before powe...

Страница 24: ... 3 note C from the probe and stir freeze wire and from the insert STAT test well Then manually rotate the operating head forward to Figure 4 Cassette Deck A H I J K L M B C D E F G A Heat Transfer Fluid Port B Numbered Cassettes in running position C Input Deck D Cassette Pusher E Supervisor Operator Keyswitch F Probe Wipers G Insert STAT Test Well H Operating Head I Head Position Adjuster J Drip ...

Страница 25: ... provided with the instru ment NOTE Save and store the solid fluid ship cap If the instrument is returned for any reason the vented fluid fill plug must be removed and the solid fluid ship cap reinstalled as part of the preparations for shipment Step 4 Load printer paper A roll of thermal printer paper and a paper roll holder are supplied with the instrument These must be installed in the printer ...

Страница 26: ...ON If the paper is not carefully centered as it is being drawn into the printer its edges may fray or tear causing the paper to jam in the printer If the printer paper becomes jammed under the print head a protective circuit will shut off the printer with out deactivating the display or the rest of the instrument To reactivate the printer shut off the 3900 and then clear the paper jam and turn the...

Страница 27: ... Soft no lint non ionic paper tissues are needed for cleaning the sample probe prior to testing Please be sure that you have an ade quate supply on hand before attempting to run tests on your instru ment Clean dry 0 2 to 0 3mL pipet Step 6 Power up your instrument When the instrument has been unpacked and installed as recommended in steps 1 5 it may be started as follows 1 Make sure the power cord...

Страница 28: ...ide to facilitate any necessary service If the instrument does not contain heat transfer fluid Fluid Required is briefly displayed then Fluid Level Empty is dis played See step 7 for filling instructions Step 7 Install heat transfer fluid CAUTION The heat transfer liquid contains hazardous chemicals Consult the material safety data sheet MSDS and use appropriate personal protective equipment 1 Loc...

Страница 29: ... drain port press and hold the reservoir pump push button for approximately 30 seconds to prime the heat transfer fluid flow through the freezing chamber 7 Remove the funnel and close the heat transfer fluid port by installing the vented fluid fill plug 8 A spare supply of heat transfer fluid should be ordered immediately Heat transfer fluid is gradually expended during operation because a small a...

Страница 30: ...h the cas sette identification number facing you 3 Press START While the self diagnostic test is running Running Diagnostics is dis played When the 3900 has completed the self diagnostic test Osmometer Ready is displayed and the instrument is ready for regu lar testing Please turn to Chapter 2 for operating instructions If the probe data has been lost from memory Reset Probe Configuration will be ...

Страница 31: ... body fluids are treated as if known to be infectious Use per sonal protective equipment such as gloves gowns etc to pre vent exposure Store biohazardous materials in regulated waste containers and dispose of these materials in a safe and acceptable manner that is in compliance with all country state and local requirements CAUTION The heat transfer fluid contains hazardous chemi cals Consult the m...

Страница 32: ...is microprocessor controlled instrument consists of auto matic sample holding and centering devices a precision digital ther mometer cooling and freezing chambers measurement and control cir cuitry a keypad and a message display panel These basic systems include the following parts and controls Power Module Figure 6 Contains the following power components Power Switch Press for power on O for powe...

Страница 33: ...transmits a significant amount of information via the commu nication port Almost every item of information displayed by the instru ment can be transmitted over the port including test results all error messages and most display data from the TEST menu The serial communication port conforms to the DTE EIA 232 V 24 RS 232 standard and its pin assignments are shown in table 2 Reservoir Pump Pushbutto...

Страница 34: ...as no effect until the instrument has completed the power up diagnostic checks If the CALIB button is pressed after the instrument has completed the power up diagnostic checks and the supervisor operator keyswitch is in the operator position a Supervisor Key Needed Press STOP to Continue message is continuously scrolled across the display until STOP is pressed Sample Tube Cassettes Figure 4 Other ...

Страница 35: ...control Contains a small amount of heat transfer fluid for optimum cooling capacity Heat Transfer Fluid Pre cooling Chamber inside Thermoelectrically pre cools heat transfer fluid for faster operation Operating Head Figure 4 Contains an electrically vibrated stir freeze wire an ultra stable ultra precise thermistor sample probe and devices to automatically center the thermistor in the sample Picks...

Страница 36: ...n required and the results of tests The messages are dis played either in English French German or Spanish as selected via the SETUP menu the default language is English The keypad contains spill proof delineated pressure buttons for operator input to the microprocessor Switch activation requires firm finger pres sure to the center of the pad When pressed properly the START STOP INSERT RECALL SETU...

Страница 37: ...Activates a test menu for checking selected components and subsystems see Test CALIB Activates calibration prompts for calibration of the instrument Pressing STOP during a calibration test will cancel recalibration and retain the previous cali bration see Calibration Numbers 1 Allow prompted numeric operator input as required during SETUP CALIB through 0 and TEST procedures and Allow stepping forw...

Страница 38: ...as been set up at the factory for normal operation but the SETUP menu may be required for changing the date and time or cus tomizing the setup parameters for your own individual needs NOTES a The supervisor operator keyswitch must be in the supervisor vertical position to enable the use of the SETUP menu If the SETUP button is pressed while the supervisor operator keyswitch is in the operator posi...

Страница 39: ...two ways to select a SETUP menu item direct selection and sequential selection described as follows 0 Select Setup Item This menu item is displayed first when SETUP is pressed and allows either direct selection or sequential selection of a menu item 21 Instrument Operation 0 Select Setup Item 1 Set Block Bin 2 Set Sample Bin 3 Select Batch 4 Select Barcode 5 Set Stir Amplitude 6 Set Date Time 7 Se...

Страница 40: ...or stepping is available from any menu position When the desired menu item is displayed press the START button to select the item display the current setting and enable changing the setting The current setting is indicated either by the symbol or a numer ic value and may be changed by pressing or or using the item specific instructions below Press ENTER to store a new setting or STOP to restore th...

Страница 41: ...ns of the numeric key pad Press ENTER to store the new setting or STOP to restore the origi nal setting Recalibrate the instrument as instructed in Calibration 2 Set Sample Bin This menu item displays the current sample probe bin setting and allows the setting to be changed CAUTION Before changing the probe bin setting the required set ting should be determined by means of the procedure described ...

Страница 42: ...00 prompts for the batch identification number to be entered via the numeric keypad each time a batch of tests is started by pressing the START button Identification numbers are then sent to the printer and the communication port along with the test results At Select Batch press START to display ON Batch OFF The current setting is indicated by the symbol At ON Batch OFF press or to toggle the batc...

Страница 43: ...eeze wire from the side to view and estimate the sample stir vibration amplitude If properly adjusted the stir freeze wire should vibrate principally fore and aft and should never strike the probe The recommended stir amplitude adjustment procedure may be found in Troubleshooting Service Pressing increases the stir amplitude pressing decreases the stir amplitude Press ENTER to store the new settin...

Страница 44: ...m enables an audible indication At Select Beeper press START to display ON Beeper OFF The current setting is indicated by the symbol At ON Beeper OFF press or to toggle the keypad beeper on or off Neither selection will affect the invalid selection beeper except that when the keypad beeper is on an invalid selec tion will invoke two beeps when off only one Press ENTER to store the new setting or S...

Страница 45: ... setting is indicated by the symbol At ON Capture OFF press or to enable or disable com munication port data capture Press ENTER to store the new setting or STOP to restore the origi nal setting 10 Select Xon Xoff This menu item enables or disables the Xon Xoff handshaking proto col for EIA 232 V 24 communication At Select Xon Xoff press START to display ON Xon Xoff OFF The current setting is indi...

Страница 46: ...that you make this selection as internal operating parameters will be revised to optimize the instru ment s performance based upon type of sample tube Press ENTER to store to store the new setting or STOP to restore the previous setting Check the calibration recalibrate as necessary Both ranges were calibrated at the factory If switching from low to high range the Model 3900 may need time to adjus...

Страница 47: ...nge the crystallization point a At Buzz Point xxxx the present setting must be cleared before the buzz point can be changed Press CLEAR to clear the setting b Enter the desired buzz point including zeros via the numeric keypad one digit at a time from left to right Entries can be erased one digit at a time from the rightmost using the button The Model 3900 buzz points can be set between 2000 and 5...

Страница 48: ... diagnostic test which requires running a single sample as described in Installation Setup As soon as the self diag nostic test is completed Osmometer Ready is displayed The Model 3900 requires no refrigeration cool down time samples may be loaded and tested as soon as Osmometer Ready is displayed the first few samples will exhibit a slightly longer test time until the reserve heat transfer fluid ...

Страница 49: ...s may be used To achieve the performance specified we recommend that you use 0 25 mL samples For pediatric use 0 2 mL samples may be more desirable but will require somewhat more critical probe and stir freeze wire adjustment for similar precision in results Where high sample evaporation is a factor 0 3 mL samples can help maximize repeatability Osmometers should always be calibrated with the same...

Страница 50: ...nt of a sample is not within the range selected a message will be displayed requesting the range be changed Like most measuring instruments osmometers need calibration against standards Your Model 3900 was calibrated at the factory using the pro cedures described in Calibration The probe and calibration parameters are stored in parameter RAM which is powered by an internal battery when the instrum...

Страница 51: ... tests Be careful not to bend the probe or stir freeze wire 3 Select a reference solution or NaCl standard with a freezing point close to that expected for your unknown Advanced Clinitrol 290 is recommended for serum Swirl and carefully open an ampule of the reference solution or NaCl standard selected 4 Select two clean sample tubes Pipet or measure identical samples of the selected reference sol...

Страница 52: ...ontrols can provide additional quality assurance 6 Using the same techniques unknown samples may be loaded into any remaining cassette positions if desired 7 Place the first cassette in the left input deck with the labels facing you 34 The Advanced Osmometer Model 3900 User s Guide Figure 7 Operating Head Stir Freeze Wire Probe Leads Clapper Stir Freeze Wire Setscrews Tube Gripper Probe Setscrew M...

Страница 53: ...the sample at the crystallization point specified by the range and buzz point selection follow the plateau development determine the osmolality and display and record the results After completing the test the Model 3900 will return the first sample tube to the cassette wipe the probe and return to the cassette for the second tube Figure 8 shows the operating head either loading or unloading the se...

Страница 54: ... be loaded into the next 1 or 2 cassettes Pre loading more than one or two cassettes will risk evaporation and or contamination of the samples CAUTIONS a The operating head constitutes a moving part while the instrument is in operation use care to avoid injury to yourself and damage to the instrument whenever intro ducing any part of the body inside the cover during oper ation b Be careful not to ...

Страница 55: ... reused in a bar coded test batch b If either batch numbering or bar code reading has been selected and for any reason the number of samples actual ly tested does not agree with the number of samples entered at the prompt of Samples WARNING Check Samples will be displayed c Cassettes should be cleaned periodically to remove the accumulation of residual heat transfer fluid from tested samples This ...

Страница 56: ... the displayed message Stand By Pre Cool after pressing Start The first test will be delayed until the sample chamber has reached a preset tem perature This will only occur for the first test sample Testing batch numbered or bar code scanned samples If either batch numbering or bar code scanning has been enabled via the setup menu it is highly recommended that you minimize sample expo sure by pres...

Страница 57: ...he bar code reading device and scan the bar codes Note that standards and controls must also be provided with bar codes and scanned in the order in which they will be placed in the cas sette After each group of 10 bar codes scanned the operator will be prompted to enter the next cassette number When finished scanning the bar codes of the specified number of samples Scanning Complete will be displa...

Страница 58: ...les To avoid contamination and evaporation cover all samples not imme diately being tested Dirty wipers can affect the results Replace the probe wipers when they begin to appear dirty about every 50 tests Use the same sample size consistently during calibration and testing Samples should not be rerun use duplicate samples for repeats The first reading in any given period of operation may be slight...

Страница 59: ... please call or write your dealer or Advanced Instruments immedi ately for Hot Line service as instructed in Troubleshooting Service STAT tests A feature of the Model 3900 is the capability to deliberately interrupt a set of tests insert a single STAT test then automatically finish the orig inal test sequence To interrupt a test set for a single priority STAT test press INSERT The INSERT button wi...

Страница 60: ...d and the instrument reverts to the standby mode and displays Osmometer Ready When the recall button is pressed at Osmometer Ready Recall 1 Run is displayed Only the results from the last run are available if barcoding has been selected but if barcoding was not selected the operator may either recall the last run accomplished or may select up to 9 runs by means of the numeric keypad or the or butt...

Страница 61: ...ormally distributed about a mean 1 S D about the mean denotes the range of values within which 68 of the measurements will fall 2 S D 95 By contrast accuracy is a measure of how close to the true value a method or measuring device has come One can repeat without being accurate but one cannot be accurate without repeating well Standards and controls To be accurate all measurements ultimately must b...

Страница 62: ...fied in your area but are not quality controlled by Advanced Instruments The Advanced 3LA028 Osmolality Linearity Set provides two 5 mL ampules each of 100 500 900 1500 and 2000 mOsm NaCl standards for verifying osmometer linearity in accordance with CLIA guidelines Please check with your associations and regulatory bodies for their guidelines and or requirements Maintenance of standards Doubts ab...

Страница 63: ...nce 3 Instrument accuracy is determined by comparing your results on precisely known standards and or controls with their specified val ues The instrument accuracy is adjustable if it is unsatisfactory it should be corrected by recalibrating as instructed in Calibration However it has been shown that frequent unnecessary recalibration will introduce inaccuracy The calibration of a freezing point o...

Страница 64: ...46 The Advanced Osmometer Model 3900 User s Guide Notes ...

Страница 65: ...ally the Model 3900 accomplishes the calibration of each required range in two steps First it records the raw means of the results of freezing point tests on standards at each of the two calibration levels in the range selected When five calibration tests have been acceptably completed at each calibration level the instrument will calculate and store the mean of the raw results of the closest test...

Страница 66: ...mOsm Advanced Osmometer Standard for the low range or 1500 and 3000 mOsm for the high range and place them in that order in an automatic calibration cassette and place the cassette in the left input deck CAUTION To avoid calibration errors due to contamination and or evaporation the automatic calibration cassette should be run as soon as possible after loading samples and preferably it should be r...

Страница 67: ...e before testing In an extremely dry room high range NaCl osmometer standards will evaporate and increase in osmolality very rapidly For reliable calibration under these conditions it may be necessary to pipet no more than one or two samples at a time and place the freshly pipet ted samples in the calibration cassette just in time to be tested Good laboratory techniques as recommended in Chapter 2...

Страница 68: ...50 The Advanced Osmometer Model 3900 User s Guide Notes ...

Страница 69: ...ic shock excessive tem peratures or mechanical hazards Performing service or maintenance not detailed in the User s Guide with or without a Service Manual should only be done by a qualified service technician Never restrict airflow into or out of the product Occasion ally check the air vents for blockage Wipe the exterior of the product with a soft damp cloth as needed Using cleaning products othe...

Страница 70: ...rotection procedures The product is equipped with operator accessible fuses If a fuse blows it may be due to a power surge or failure of a component Replace the fuse only once If the fuse blows a second time it is probably caused by failure of a component part If this occurs refer service to qualified service personnel Always replace the fuse with one of the same rating voltage and type Never repl...

Страница 71: ... symptoms of your problem ready You should use a telephone as close to your instrument as possible to facilitate mak ing recommended diagnostic checks If you need to order parts or service a purchase order from your purchasing agent will be necessary After Hot Line diagnosis your service technician may assist you in mak ing minor repairs over the phone providing you with recommended parts or part ...

Страница 72: ...ng in place into the sample freezing chamber with enough force to hold both in place during shipment Doing so will prevent any instrument damage from fluid remaining in the cooling system 6 Place a clean dry probe wiper over the tips of the probe and stir wire as shown in figure 3 7 Manually rotate the operating head clockwise and gently lower the wiper covered probe into the insert test well 8 In...

Страница 73: ...luid should be drained and replaced at least every two months as follows 1 Connect the quick connect connector and short length of tubing supplied with the 3900 to the reservoir drain port in the back panel of the instrument 2 Place a container under the tubing to catch the draining heat transfer fluid 3 Press and hold in the reservoir pump push button for a few moments to pump the heat transfer f...

Страница 74: ...he Advanced Model 3900 osmometer The lowest level simply involves requesting help as recommended in Obtaining Service but it will facili tate obtaining help if you can do some simple guided troubleshooting Before assuming that the instrument itself is faulty carefully review the operational requirements listed in the product specifications and the recommended setup and operating procedures in Appe...

Страница 75: ...hnicians If recommended in the troubleshooting table running an appropriate diagnostic menu test from those described in Test section see below may provide more information Adjustment and Replacement of Parts and Assemblies contains some additional tests and adjustment and replacement instructions The highest levels of troubleshooting and field service may require much more technical ability and p...

Страница 76: ...ed test is displayed press the START button Or at Select Test Item press the START button The display will change to Test Menu 0 From the list above type the number of the desired test on the numeric keypad The 0 on the display will change to the number typed if valid Then press ENTER The dis play will show the selected test Next press the START button The requirements are different for each test ...

Страница 77: ...in the left input deck and press the START button When START is pressed the A D tests display the target cooling block temperature the channel being read and the current channel reading or duty cycle The display is in the form off Blk NNNN NN ohm where off indicates that a target temperature has not yet been selected Blk indicates that the block probe chan nel is being tested NNNN NN is a numeric ...

Страница 78: ...tem enables visually and aurally checking the stir and freeze vibration amplitudes Please refer to Adjustment and Replacement of Parts and Assemblies for instructions 3 Probe Bin Test This test is used to determine the resistance and bin number of the sample probe at a specific temperature 0 093 C It is essentially the same as any other sample test except that the sample probe resis tance and corr...

Страница 79: ...sed as a troubleshooting aid in con junction with the sample probe checks described in Adjustment and Replacement of Parts and Assemblies Record the sample probe resistance and bin number in the service log at the end of this user s guide and or other convenient location then press the STOP button The display will revert to Probe Bin Test Press SETUP and use the procedure described in Installation...

Страница 80: ... display START Loop Ready Press the START button to run the test The instrument will begin to continuously send characters to the communication port and display a count of the number of characters sent and the number of overrun framing errors that occur or will display the message Serial Loop Failed After a serial loop failure the test may be rerun by pressing the START button Press STOP to exit t...

Страница 81: ...s the button to cause the beeper to beep repeatedly press the button to stop the beeper Press STOP to exit to the test menu 7 Keypad Test This test enables checking each button on the keypad as well as the lamps of each illuminated button As each button is pressed its name should be displayed and the lamp of each illuminated button should light Press the STOP button to exit to the test menu after ...

Страница 82: ...ette Index Test This test continuously cycles the cassette index mechanism On entry START Test STOP is displayed Press START to begin the test If a cassette is in position to be engaged by the cassette index mech anism it will be moved forward one position per cycle unless STOP is pressed Press STOP to exit to the test menu 10 Cassette Pusher Test This test continuously cycles the cassette pusher ...

Страница 83: ... second test mode rotates the operating head between the probe wipers the sample tube loading position the INSERT test well the freezing chamber the sample tube loading position and the INSERT test well continuously in that order The head only pauses at the sample tube loading position at the INSERT test well and the freezing chamber it descends nearly to the sample tube To help avoid damage the o...

Страница 84: ...ach cassette position will be indexed and displayed Each sample tube will be picked up rotated and returned to the cassette Any empty cassette position will be so indicated on the printer tape This cycle will continue until STOP is pressed all cassette positions have been tested or a sample handling error occurs If an error occurs the test will pause and an appropriate message will be displayed an...

Страница 85: ...ers firmly straight in as far as they will go 2 Fuse Replacement The power switch and fuse holder are located beside the power cord connector on the back panel of the instrument Switch the power switch to the off O position and disconnect the power cord Use a small flat bladed screwdriver or similar tool to pry open the fuse holder door Visually check for a blown fuse If there is any doubt test th...

Страница 86: ...etected via close visual inspection or disconnecting the connector and checking the probe resistance This can be done in place without disturbing the physical adjustment of the probe The following procedure is recommended for testing and or replac ing the sample probe please refer to figure 7 for the locations of the parts named in this procedure a Turn the 3900 power off and disconnect it from th...

Страница 87: ...placement a The 3900 should be disconnected from the power source the back cover removed and the sample probe disconnected as instructed in Sample Probe Checks item 3 a through 3 c b Push pull out the pivot plate pin and remove the pivot plate see figure 7 c Pull the tube gripper see figure 7 down and off of the mandrel leave the large coil spring in place d Loosen the probe setscrew see figure 9 ...

Страница 88: ...structed in item 7 and recalibrate as instructed in Chapter 4 5 Probe and Stir Freeze Wire Alignment Proper alignment and adjustment of the probe and stir freeze wire are of utmost importance to assure accuracy precision and proper operation of the instrument 70 The Advanced Osmometer Model 3900 User s Guide Figure 9 Tube Gripper Mandrel Probe Stir Freeze Wire and Alignment Tool End View Tube Grip...

Страница 89: ...ar end of the probe and stir freeze wire alignment tool firmly up against the bottom of the inner core of the mandrel with the footed face of the alignment tool toward the stir freeze wire as shown in figure 9 c Align the probe against the raised V centerline on the footed face of the alignment tool see figure 10 d As necessary loosen the probe setscrew manually raise or lower the probe within the...

Страница 90: ...EP as shown in figure 10 g Reverse the steps described in Sample Probe Checks item 3 a through 3 c to restore the instrument to operating condition Each time the 3900 is turned on it follows the power up sequence described in Installation Setup to the point where Press START to Continue scrolls across the display h Always check and readjust the stir freeze amplitude as necessary after aligning the...

Страница 91: ... Select Test Item e At Select Test Item press the START button The display will change to Test Menu 0 f At Test Menu 0 press the 2 button then press ENTER The display will change to Stir Freeze Test g At Stir Freeze Test press START The display will change to stir freeze h At stir freeze a freeze pulse will occur each time the button is pressed Watch the tip of the stir freeze wire and press The f...

Страница 92: ...y or frothing which may make it impossible to reliably determine the freezing point of certain samples k If the stir vibration amplitude is not correct press STOP twice to exit the TEST menu and press SETUP to enter the SETUP menu l At Select Setup Item press the START button The display will change to Setup Menu 0 m At Setup Menu 0 press the 4 button then press ENTER The display will change to Se...

Страница 93: ...t so great as to produce froth or bubbles If froth or bubbles are produced decrease the stir amplitude setting 5 digits lower than that at which bubbles just begin to appear p When the stir amplitude is correct you may wish to record the stir amplitude xx value in the service log at the end of this user s guide for reference Press ENTER to store the new setting The display will change to Set Stir ...

Страница 94: ...ct Test Item c At Select Test Item repeatedly press the button until the dis play reads Probe Bin Test d At Probe Bin Test press START The display will change to Start Ready e At Start Ready pipet or measure samples of bin setting fluid into three clean sample tubes and place them in the first three positions of a cassette f Place the cassette in the left input deck and press the START button The ...

Страница 95: ...g j If the current sample probe bin setting is the same as that just determined simply press the STOP button twice to return to Osmometer Ready If the current sample bin number setting is not the same as that just determined enter the correct bin num ber by means of the numeric keypad then press ENTER The display will change to Set Sample Bin Press STOP to return to Osmometer Ready k Press the CAL...

Страница 96: ...as it is from the side walls B The flange of the mandrel must be flush with the head chassis C The stir freeze wire should be 1 1 5 mm 1 32 1 16 away from the man drel at this point See bottom view D The stir freeze wire should be about 1 mm 1 32 away from the probe Bend the wire where it changes diameter if necessary MANDREL BOTTOM VIEW PROBE MANDREL STIR WIRE A B C D Figure 11 Mandrel Probe and ...

Страница 97: ...ty checker or replacement fuse to check continuity if there is any doubt Check the values marked on the fuses For 100 130V operation they should be 2 Amp fuses for 200 250V operation they should be 1 Amp fuses Symptom Check Suggested Action 1 Have the power restored 2 Make sure the power cord is firmly plugged into both the wall outlet and the socket on the back of the instrument 3 Replace the fus...

Страница 98: ... Visually check for a cas sette in the wrong position or other visible mechani cal cause Reported when buzz point is changed Buzz setting can change cali bration Symptom Check Suggested Action If the problem persists see Chapter 5 for further assis tance If the problem persists see Chapter 5 for further assis tance Repeat calibration Repeat calibration 1 Clear the cause of the problem if visible a...

Страница 99: ...ont right side Symptom Check Suggested Action Retry the test If the problem persists see Chapter 5 for further assis tance Use a cassette not previ ously used in this run or start another batch If the problem persists see Chapter 5 for further assis tance If the problem persists see Chapter 5 for further assis tance A wiring or circuit board error is indicated If the problem persists see Chapter 5...

Страница 100: ...align the probe and stir freeze wire as necessary If the problem persists see Chapter 5 for further assis tance 1 Clear and discard the stuck sample tube or other visible cause of the malfunction 2 Check that the probe and stir freeze wire are still in proper position 3 Press STOP to reset the instrument 4 Remove the interrupted cassette To finish testing it remove already tested samples and place...

Страница 101: ...position in the left cassette deck to begin testing or calibration see figure 4 Switch the instrument off then on Procedure Symptom Check Suggested Action Re enter recall with fewer runs Try to avoid introducing un scanned cassettes dur ing batch test runs Place a loaded cassette in proper position in the left cassette deck before press ing START to begin testing or calibration Test another aliquo...

Страница 102: ...followed by Reset Probe Configuration see Chapter 5 for further assis tance If followed by Reset Probe Configuration see below If the problem persists see Chapter 5 for further assis tance If the error message repeats after successful re calibration see Chapter 5 for further assistance If not followed by Select Test Item see Chapter 5 for further assistance If followed by Select Test Item see belo...

Страница 103: ...Did you wire loop back connector correctly 3 Try lower baud rate Symptom Check Suggested Action continued 3 Reset the stir amplitude as necessary to the num ber recorded in the ser vice log 1 Test another aliquot of that sample at the range indicated 2 Change range as indicat ed 3 Recalibrate or dilute and rerun the sample as nec essary Recalibrate unless running a 0 mOsm sample Replace the sample...

Страница 104: ...figura tion correct Symptom Check Suggested Action 1 Test another aliquot of that sample in a clean sample tube 2 Try testing samples known to be within the range of the instrument 3 Make sure samples con tain no bubbles or froth before testing Decrease the sample stir ampli tude if necessary to avoid generating bubbles before the freeze buzz occurs 4 The 3900 is not designed to test thick or very...

Страница 105: ... matter between the stir wire clapper and coil 6 Does the clapper and or the stir wire coil show excessive wear Symptom Check Suggested Action 1 Clean all debris from the magnetic gap 2 Tighten both stir freeze mounting screws firmly see figure 7 3 Correct the stir freeze wire alignment as neces sary 4 Correct the stir freeze amplitude as necessary 5 Use a paper towel to clean between these parts ...

Страница 106: ...1 Replace the probe wipers and test another aliquot of that sample in a clean sample tube Check correct the probe bin numbers 2 Correct the stir freeze wire alignment and adjustment as necessary 3 Try to minimize the effects of materials that tend to cause premature crystallization 4 False pre freeze errors may be reported if the instrument cannot main tain a cooling sample rate of greater than 20...

Страница 107: ...er Switch the instrument off then on Check the key switch position Symptom Check Suggested Action If the error message does not persist and other mes sages are not displayed ignore this message Replace the sample probe If the error message does not persist and other mes sages are not displayed ignore this message Replace the sample probe Remove the misplaced tube check the probe and stir freeze wi...

Страница 108: ... ation or at the completion of a batch bar code run if samples recorded does not match that entered for batch bar code run Symptom Check Suggested Action If the error message does not persist and other mes sages are not displayed ignore this message Replace the sample probe 1 Prepare another sample and repeat the test 2 Check correct the block probe bin number 3 If the problem persists see Chapter...

Страница 109: ...Osm kg H2O Ranges Low 0 to 1550 mOsm kg H2O High 1450 to 4000 mOsm kg H2O Resolution 1 mOsm kg H2O Communications On board printer DTE EIA 232 V 24 RS 232 serial port and optional barcode scanner Performance at Reference Conditions1 Linearity Low Range Less than 0 5 from a straight line between 0 and 1500 mOsm kg H2O High Range Less than 1 0 from a straight line between 1500 and 4000 mOsm kg H2O R...

Страница 110: ...y 3 to 10 minutes Maximum throughput may require up to 30 minutes of equilibration time from power on Test Time 90 seconds per sample typical Sample Approximately 30 samples per hour at 23 C 73 F less at Throughput higher ambient temperature and in high range Dimensions inches centimeters Width 21 5 54 6 Depth 20 0 50 8 Height 21 5 54 6 Weight pounds kilograms Net 66 0 30 0 Shipping 105 0 47 7 War...

Страница 111: ...s product Consult the distributor or dealer for more information and documen tation Connections to this product must be made with shielded cables Use of non shielded cables may violate RFI EMI limits Symbol Conventions This symbol indicates conformity to relevant European direc tives This symbol indicates the product was tested to conform to rele vant Canadian and U S safety standards by Intertek ...

Страница 112: ...n of This product meets the intent of Directive 89 336 EEC Conformity EMC for Electromagnetic Compatibility Compliance was demonstrated using the following standards as listed in the Official Journal of the European Communities Consult the Declaration of Conformance certificate shipped with the product for the latest update EN 61326 1998 Group 1 Class B Electrical Equipment for Measurement Control...

Страница 113: ... Consult the Declaration of Conformance certificate shipped with the product if required for the latest update EC Declaration This product meets the intent of Directive 2002 96 EC of Conformity as amended by 2003 108 EC for Waste Electrical and WEEE Electronic Equipment WEEE Consult the Declaration of Conformance certificate shipped with the product if required for the latest update ...

Страница 114: ...96 The Advanced Osmometer Model 3900 User s Guide Notes ...

Страница 115: ...onsibilities which constitute contractual warranties and conditions between the seller and the user for the maximum benefit and useful ness of the instrument ADVANCED INSTRUMENTS WARRANTS THAT IT 1 Has produced equipment equal to or exceeding that of any competitive product in the same price range in standards of design material and workmanship ...

Страница 116: ...d without written approval from Advanced Instruments 4 Advanced Instruments will be notified immediately if any injury occurs in any association with the instrument and will be allowed prompt and thorough exami nation of the instrument in question 5 Advanced Instruments will be held harmless in cases of injury arising see definitions below a Beyond the useful life of the equipment b From unreasona...

Страница 117: ...corrected repairs d Only for the purpose stated in the instructions provided with the instrument e In which the equipment has been maintained according to the instructions provided 3 Immediate notification is a Recognition that time is of the essence when any accident mal practice or product liability arises which involves Advanced Instruments equipment b Notification to Advanced Instruments immed...

Страница 118: ...The Advanced Osmometer Model 3900 User s Guide 100 Notes ...

Страница 119: ...ine Feed 0A Hex Note that your instrument is only capable of outputting information At this time there is no protocol for talking to the instru ment Sample RS 232 Setup As a typical example of a communications program setup the following instructions will describe the process necessary for using your instru ment in conjunction with Hyperterminal for Windows 95 or later oper ating systems and a nul...

Страница 120: ... bits 1 Flow control None 5 Select the Settings menu 6 Chose TTY for the Emulation option 7 Select ASCII setup 8 Set the Sending option to Echo Typed Characters Locally 9 Set the Receiving option to Wrap Lines that Exceed Terminal Width You should at this point see all instrument output in the Hyperterminal window of your computer screen 102 Null Modem Cable Shield connects to metalized connector ...

Страница 121: ...ions Appendix F On Off Feed Interrupt Test Start Stop Record Review Setup Calibration Cancel Delete Functional Arrow Printer Enter RS232 Bar Code Attention Caution Hot Surface Dangerous Voltage Lifting Hazard Calibrator ...

Страница 122: ...Fragile Irritant Keep Dry Date Manufactured Sterile Non Sterile Serial Number Solution Do Not Open Top Handle With Care Toxic Use Blade To Open Do Not Re Use For In Vitro Diagnostic Use European Conformity Temperature Limit See Instructions Lot Number Use By Expiration Date Authorized Representative Part Number ...

Страница 123: ...itions Sufficient for x Tests Open Here Low Fluid Level Keep Hands Clear Manufacturer Latex Free Diluent See Instructions for Temperature Guidelines Potential Puncture Hazard Electronic Equipment Dispose of Properly x ...

Страница 124: ...106 The Advanced Osmometer Model 3900 User s Guide Notes ...

Страница 125: ...ince regulations governing the disposal of your instrument and acces sories may vary depending upon your geographic location the following guidelines are provided to assist you in identifying the options available to you once the decision has been made to replace or dispose of this product Contact the supplier who sold you the product Whether this was Advanced Instruments itself or one of its auth...

Страница 126: ...ts can be sent Contact Advanced Instruments Hot Line Service 800 225 4034 toll free within the USA and Canada after normal business hours dial extension 2191 US 781 320 9000 elsewhere 781 320 0811 fax Service personnel will provide you with contact information for local disposal or instructions for returning the product directly to Advanced Instruments 108 The Advanced Osmometer Model 3900 User s ...

Страница 127: ...109 Model 3900 Serial Number Software Revision Sample Probe Number Block Probe Number Service Log Appendix H Date Problem Symptom Action ...

Страница 128: ...The Advanced Osmometer Model 3900 User s Guide 110 Date Problem Symptom Action ...

Страница 129: ...libration 47 notes 48 procedure 47 standards vii 31 cassette deck 6 index test 64 pusher test 64 Clinitrol 32 concentrative properties xiv controls 43 D data capture 27 date time 25 diagnostics 12 display print test 63 E error messages 79 event record 81 F freezing point xiv fuse replacement 67 fuses 2 67 H head rotation test 65 head up down test 63 heat of fusion xv heat transfer fluid 10 31 Hype...

Страница 130: ...ements 56 osmolality xvii osmolarity xvii osmometry xvii P pipette 12 power switch 9 printer 7 18 probe adjustment 70 probe bin test 60 product test 30 Protinol 34 44 Q quality control 43 implementation 45 R recall 42 range 28 repeatability 40 43 reservoir status 30 RS 232 port 101 S sample bin 23 pre freeze 88 preparation 32 sample probe 9 bin setting 76 checks 68 replacement 69 sample sizes 31 s...

Страница 131: ...43 STAT tests 41 stir amplitude 25 stir freeze amplitude adjustment 72 test 60 wire adjustment 70 supercooling xv supervisor operator keyswitch 15 T test mode options 58 test time out error 90 troubleshooting 56 59 tube transport test 66 W warranty 97 X Xon Xoff 27 Index 113 ...

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