Zeiss Primostar 3, Instruction Manual

Page: 80 / 112

5 First Operating Steps | 5.8 Adjusting the Transmitted-Light Simple Polarization Contrast ZEISS
5.8 Adjusting the Transmitted-Light Simple Polarization Contrast
Prerequisite ü The simple polarization contrast method on the microscope requires a stand with Full-Köhler
equipment.
ü The polarizer plate is inserted into the luminous-field diaphragm in the correct position, see
Installing the Polarizer Plate (Full Köhler Stand) [
}
49] .
ü
The analyzer plate is inserted into the stand in the correct position, see Installing the Analyzer
Plate [
}
49] .
Procedure 1. Adjust the microscope as you would for transmitted-light brightfield, see Adjusting the
Transmitted-Light Brightfield on the Full-Köhler Microscope [
}
69] .
2. Place the specimen to be examined under po-
larized light on the stage and fix it using the
spring lever of the specimen holder.
5.9 Adjusting the Reflected-Light Fluorescence
Prerequisite
ü The reflected-light fluorescence method requires the microscope with Reflected-Light Fluores-
cence Illuminator iLED
Procedure 1. Adjust the microscope as you would for transmitted-light brightfield, see Adjusting the
Transmitted-Light Brightfield on the Full-Köhler Microscope [
}
69] .
2. Turn the nosepiece to move the objective for
fluorescence application into the light path
(e.g. objective 40x).
80 Instruction Manual ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7011-111_en