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START-UP
ZEISS
Mounting optional components
Axioscope 5/7/Vario
74
430035-7344-001
03/2018
3.2.4
Changing the filters in the reflector
module FL P&C
•
Remove the reflector module FL P&C
(
3
) from the reflector turret (see also
•
Unscrew the adapter ring (
1
) with the
mounting plate included with the tools
(
6
).
•
Turn over the reflector module and let the filter
(
2
or
5
) drop onto a soft surface.
•
Insert the emission filter (
2
) or the
excitation filter (
5
) respectively at the
•
Fix the filters with the corresponding adapter
ring (
1
).
NOTE
When LED modules (423052-9593-000 for 385 nm, 423052-9573-000 for 470 nm, 423052-
9562-000 for 505 nm, 423052-9602-000 for 565 nm and 423052-9522-000 for 625 nm) are
used for fluorescence, the respective excitation filter is pre-installed in the LED module instead
of the reflector module.
The emission filter and excitation filter usually have
a name and an arrow on their rims. The arrow
indicates the direction of insertion and must always
point to the inside of the reflector module (see
arrows in
Fig. 3-41). To minimize the image offset
in multiple fluorescence images, the emission filter
may have another mark to level the point angle.
When mounting the appropriate emission filter in
the according reflector module, the mark should
be aligned with the orientation groove
(
4
). This guarantees that the point angle
in the reflector modules acts equally and
compensates or minimizes the image offset –
already low in ZEISS filter sets – even further.
When mounting a filter without any orientation
marks (arrow), we recommend proceeding as
follows:
Filters with reflective, di-electrical coatings are
mounted with the reflective coating (
6
)
facing outward (in relation to the reflector module)
in excitation filters (
5
) and facing inward
in emission filters (
2
).
The reflective coating (
4
) of the color splitter (
3
) faces down when the splitter is
mounted.
The arrows (
7
) in the figure mark the path of the illumination beam or the imaging beam.
Fig. 3-41
Changing the filters in the reflector
module FL P&C
Fig. 3-42
Mounting the filters and the color
splitter