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OPERATION
Axioscope 5/7/Vario
Illumination and contrast methods in reflected light …
ZEISS
03/2018
430035-7344-001
123
Fig. 4-20
Components for reflected light fluorescence
•
Keep the light path in the reflected light illuminator blocked via the elimination position of the filter
slider for reflected light (
2
).
•
Switch on the Colibri 3 illuminator by pushing the
RL
button (Fig. 4-20/
7
).
•
Press the
Intensity/LM
button (Fig. 4-20/
6
) briefly for less than 1.5 seconds repeatedly to activate the
required LED module or all LED modules of Colibri 3 together. The indicator light of the respective LED
module on the Colibri 3 lights up when this module is switched on.
NOTE
If using the HBO 100 illuminator, switch on the external power supply and let it warm up to
operational temperature for about 15 minutes.
•
On the reflector turret/slider (
8
), choose the FL P&C reflector module with the desired
fluorescence filter combination (depending on the excitation mode) and switch it on.
•
Now unblock the light path in the reflected light illuminator with the filter slider for reflected light
(
2
).
•
If necessary, adjust the FL attenuator to 100% transmission in order to facilitate locating fluorescence
signals. Reduce the transmission later to preserve the sample.