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OPERATION
Axioscope 5/7/Vario
Illumination and contrast methods in transmitted light …
ZEISS
03/2018
430035-7344-001
109
4.2.7.4
Conoscopy with intermediate plate and Bertrand lens slider
(1) Microscope configuration
−
Recommended strain-free, high-quality objectives:
EC Plan-Neofluar 40x/0.9 Pol objective or
EC Plan-Neofluar 100x/1.30 Oil Pol objective or
EC Epiplan-Neofluar 50x/0.8 Pol objective or
EC Epiplan-Neofluar 100x/0.9 Pol objective
−
Axioscope stand for polarization with intermediate plate
−
Pol rotary stage
−
Pol binocular photo tube
or
eyepiece with crossline micrometer 14:140 (454060-0000-000)
and
adjustment aid for polarization microscopy (453679-0000-000)
−
Achromatic-aplanatic 0.9 BF Pol condenser or 0.9 Pol condenser
−
Bertrand lens slider in intermediate plate
−
D analyzer module in reflector turret
−
D polarizer (rotatable or fixed)
(2) Setting the microscope for conoscopy using the intermediate plate and the Bertrand lens
slider
•
Set the microscope as for transmitted light brightfield microscopy using the KÖHLER method (see also
section 4.2.1).
•
Put the specimen onto the stage and focus on it.
•
Move the polarizer and the D analyzer module into the light path.
•
First, align the crossline micrometer 14:140 or the eyepiece reticle to the vibration direction of the
polarizer using the Pol adjusting tool sample. Please refer to section 4.2.6.2.
NOTE
If you do not use the Pol binocular photo tube for your work, you must realign the reticle
after changing the inter-pupillary distance on the binocular tube.
•
Remove the Pol adjustment tool.
•
Put the specimen onto the stage and focus on it.
In the case of uniaxial crystals, the most favorable orientation for conoscopic viewing is obtained with
those specimen features (e.g. of a thin section) that in orthoscopic viewing change the brightness as little
as possible upon rotating the stage. In this case, the direction of viewing and the optical axis are
±
parallel. The same refers also to biaxial crystals, if they are viewed along or approximately in the
direction of one of the two optical axes.
•
Move a selected crystal to the center of the reticle.
•
Swivel the front lens on the condenser into the light path.