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OPERATION
ZEISS
Illumination and contrast methods in reflected light …
Axioscope 5/7/Vario
116
430035-7344-001
03/2018
4.3.3
Setting reflected light DIC and reflected light C-DIC
(1) Application
The reflected light DIC and the reflected light C-DIC methods (DIC = Differential Interference Contrast;
C-DIC = Differential Interference Contrast in circular polarized light) are used for the high-contrast
imaging of phase objects, i.e. objects which predominantly change the phase of the light, in contrast to
the amplitude objects.
(2) Instrumentation
−
Axioscope with pre-installed LED 10 W illuminator or optionally used with the HAL 100 halogen
lamp mounted at the back of the upper part of the stand
−
Objectives: EC Epiplan-Neofluar, Epiplan with the additional label "DIC" or "Pol"
−
DIC slider, fitted to the objective engraved on the top surface of the slider with amplification and
aperture or 6x20 mm C-DIC slider (in connection with the DIC P&C reflector module, compensator
slot 6x20 mm)
(3) Reflected light DIC, preferably for objectives Epiplan 5x to 100x and LD-Epiplan 10x to 100x
•
Adjust the microscope for reflected light brightfield microscopy as described in section 4.3.1. Open the
field diaphragm enough so that the edge of the diaphragm lies just barely outside of the edge of the
field of view to avoid reflections.
•
Swing the DIC P&C reflector module on the reflector turret/slider into the beam path.
•
Swing in the objective position with the DIC position on the nosepiece.
•
Slide the DIC slider into the slot of the nosepiece (above the objective).
•
Place the sample on the stage, then focus and turn the mechanical stage so the structure of interest is
visible with maximum contrast.
•
The contrast can be optimized with the knurled button on the DIC slider.
(4) Reflected light C-DIC
•
Adjust the microscope for reflected light
brightfield microscopy.
•
Swing the C-DIC P&C reflector module into the
beam path.
•
Slide the C-DIC slider (
2
) into the
6x20 mm compensator slot (
1
).
•
Place the sample on the stage, focus and turn
the control knob (
4
) on the C-DIC
slider so the structure of interest is visible with
maximum contrast. No further stage rotation is
necessary.
•
The contrast can be optimized by adjusting the
control knob (
3
) on the C-DIC slider.
Fig. 4-16
Compensator slot 6x20 mm with
C-DIC slider 6x20 mm