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OPERATION
ZEISS
Illumination and contrast methods in reflected light …
Axioscope 5/7/Vario
124
430035-7344-001
03/2018
•
Remove an eyepiece from the tube and adjust the aperture diaphragm by sight.
•
Open the aperture diaphragm (
3
) enough to see the whole objective exit pupil.
•
Replace the eyepiece in the tube and close the field diaphragm (
4
) enough to make it visible
in the field of view.
•
Using both centering screws (
5
), center the field diaphragm on the edge of the field of view.
•
Open the field diaphragm enough to make it just disappear behind the edge of the field of view, or, if
you are using a sample which might bleach out, reduce the field diaphragm for the field of view.
•
Focus the sample again and optimize the collector position of the HBO 100 (if used). Adjust the
collector so that the reflector module of the short-wave excitation illuminates the field of view
consistently. A correction of the collector position is not necessary in modules with longer-wave
excitation.