Zeiss Axioscope 5, Instruction Manual

Page: 110 / 204

5 Operation | 5.5 Setting Up for Reflected Light Techniques ZEISS
3. Slide the according DIC slider
1
into the slot
of the nosepiece.
1
2
4. Place the sample on the stage.
5. Focus the sample.
6. Turn the mechanical stage so the structure of interest is visible with maximum contrast.
7. Use the knurled screw
2
on the DIC slider to adjust the optimal contrast.
5.5.4 Setting Up for Reflected Light C-DIC Microscopy
For more information about the method, see chapter Reflected Light DIC and C-DIC Microscopy
[
}
59] .
Prerequisite ü A reflected light light source is installed [
}
77] .
ü
The microscope is operational.
ü C-DIC reflector module is installed.
ü A suitable objective for DIC is installed [
}
67] , e.g. EC Epiplan-Neofluar, Epiplan with the addi-
tional label "DIC" or "Pol".
ü C-DIC slider compatible with the objectives in use, is available.
ü The illumination is adjusted for reflected light brightfield microscopy [
}
105] .
Procedure 1. Swivel the C-DIC module on the reflector turret
into the beam path.
2. Swivel the DIC compatible objective into the
beam path.
110 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001