Zeiss Axioscope 5, Instruction Manual

Page: 109 / 204

ZEISS 5 Operation | 5.5 Setting Up for Reflected Light Techniques
7. Focus the sample.
9
The illumination is now adjusted for darkfield microscopy.
5.5.3 Setting Up for Reflected Light DIC Microscopy
For more information about the method, see chapter Reflected Light DIC and C-DIC Microscopy
[
}
59] .
Prerequisite ü A reflected light light source is installed [
}
77] .
ü
The mechanical stage, 75x50/240° rotatable or the rotary stage Pol is installed [
}
69] .
ü The microscope is operational.
ü DIC reflector module is installed.
ü A suitable objective for DIC is installed [
}
67] , e.g. EC Epiplan-Neofluar, Epiplan with the addi-
tional label "DIC" or "Pol".
ü DIC slider compatible with the objectives in use, is available.
ü The illumination is adjusted for reflected light brightfield microscopy [
}
105] .
Procedure 1. Swivel the DIC module on the reflector turret
into the beam path.
2. Swivel the DIC compatible objective into the
beam path.
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 109