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OPERATION
Axio Observer
Illumination and contrast techniques
ZEISS
12/2016
431004-7244-001
147
•
Set the light intensity to a comfortable level by using the illumination control wheel (Fig. 148/
32
).
•
Close the luminous-field diaphragm
(Fig. 148/
10
) until it is visible in the field of view
(not necessarily in focus) (Fig. 149/
A
).
•
Bring the edge of the luminous-field diaphragm
into focus (Fig. 149/
B
) by adjusting the height
of the condenser (Fig. 148/
13
).
•
Center (Fig. 149/
C
) the luminous-field
diaphragm using the centering screws
(Fig. 148/
8
) and open until the edge of the
diaphragm just disappears from the field of
view (Fig. 149/
D
).
•
To set the aperture diaphragm, remove one eyepiece from the eyepiece tube and adjust the aperture
diaphragm of the condenser to approximately 2/3 the diameter of the exit pupil of the eyepiece
(Fig. 149/
E
).
The settings required for optimum contrast depend on the specimen.
•
Reinsert the eyepiece and where necessary refocus on the specimen using the fine focusing drive.
•
Adjust the light intensity using the rocker switch.
The field size and objective aperture change each time the objective is changed, so that for
optimal results the luminous-field diaphragm and aperture diaphragm should be readjusted
whenever the objective is changed.
Fig. 149
Diaphragm settings in transmitted
light brightfield acc. to KÖHLER
Summary of Contents for Axio Observer Series
Page 1: ...Axio Observer Inverted microscope Operating manual...
Page 192: ......