YMC Pilot PLUS, Manual

Page: 3 / 49

YMC Europe GmbH Contents
YMC Pilot 3 / 49
PLUS
low pressure chromatography column
Contents
1 Information on manual ........................................................................................... 5
1.1 General ................................................................................................................ 5
1.2 Explanation of warnings ....................................................................................... 5
1.3 Structure of instructions ....................................................................................... 6
1.4 Guidelines ............................................................................................................ 6
1.5 Figures ................................................................................................................. 6
2 Safety ....................................................................................................................... 7
2.1 Intended use ........................................................................................................ 7
2.2 General safety instructions .................................................................................. 7
2.3 Personnel requirements ....................................................................................... 7
2.4 Personnel protective equipment .......................................................................... 8
3 Specifications and overview .................................................................................. 9
3.1 Fields of application ............................................................................................. 9
3.2 Specifications ....................................................................................................... 9
3.3 Chemical Resistance ......................................................................................... 10
3.4 Overview ............................................................................................................ 11
3.4.1 Adjustable end piece .................................................................................... 12
3.4.2 Column body................................................................................................. 13
3.4.3 Column stand................................................................................................ 14
4 Delivery .................................................................................................................. 15
4.1 Delivery contents ............................................................................................... 15
4.2 Delivery inspection ............................................................................................. 15
5 Requirements for Operation ................................................................................ 16
5.1 Pilot column set up ............................................................................................ 16
5.2 Pressure test ...................................................................................................... 16
6 Operation ............................................................................................................... 18
6.1 Removal of adjustable end piece ...................................................................... 18
6.2 Pilot column packing .......................................................................................... 19
6.2.1 Slurry preparation ......................................................................................... 19
6.2.2 Slurry column packing under flow................................................................. 20
6.2.3 Slurry column packing with sedimentation ................................................... 24
6.3 Column test and release criteria ........................................................................ 27
6.4 Pilot column unpacking ...................................................................................... 28
7 Cleaning and maintenance .................................................................................. 30
7.1 Notes for Cleaning-In-Place (CIP) ..................................................................... 31
7.2 Cleaning and maintenance plan ........................................................................ 32
7.3 Cleaning ............................................................................................................. 33
7.4 Replacement of spare parts............................................................................... 36
7.4.1 Frit replacement in the adjustable end piece ................................................ 36
7.4.2 Replacing the glass body ............................................................................. 37
7.4.3 Frit replacement column body ...................................................................... 40
8 Troubleshooting .................................................................................................... 42
9 Storage, packaging and transport ...................................................................... 44
9.1 Storage .............................................................................................................. 44
9.2 Transport............................................................................................................ 44