YMC Europe GmbH
Operation
YMC Pilot
20 / 49
PLUS
low pressure chromatography column
6.2.2
Slurry column packing under flow
The column is packed with the slurry method under flow as follows:
1. Remove the adjustable end piece Chapter 6.1, S. 18.
2. Check the drainage tubing for correct connection.
3. Check the drainage tubing outlet for correct position in the collecting vessel.
4. Connect the pump with the tubing at the column body.
5. Start the pump.
6. Fill the glass body with solvent or buffer until 2-3 cm above the frit in the
column body (bottom-up).
This ensures that no air is trapped under or is inside the frit.
7. Close the valve in the tubing on the column body.
8. Connect the pump with the adjustable end piece.
9. Flush the piston of the adjustable end piece with slurry solvent until the frit of
the adjustable end piece is wetted completely.
10. Disconnect the pump from the adjustable end piece.
11. Pour the homogenous and/or degassed slurry into the glass body.
12. Rinse the inner wall of the column body with slurry solvent for removal of
particles.
By removing particles from the inner wall of the column body, a correct seal
with the profile gasket is guaranteed.
13. Fill the pilot column up to the edge of the drain function with slurry solvent.
14. Turn the closing disc of the adjustable end piece in the UP direction as far as it
will go.
