YMC Europe GmbH
Operation
YMC Pilot
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PLUS
low pressure chromatography column
24. Start the flow (top down).
25. Increase the flow rate stepwise until the desired pressure / flow rate is
reached. Wait after every step until the pressure is constant. Afterwards, the
flow rate can be further increased.
Consider the manufacturer
’s recommendations for the packing material.
The flow rate should be considerably (> 20%) higher than the operating flow
rate.
The column bed is visually compressed.
26. Check and mark the bed length frequently.
27. If the final bed length is reached, stop the pump.
28. Turn the piston of the adjustable end piece in the DOWN direction until the
column bed is reached.
29. Start the pump.
30. Check, if the column bed is further compressed. A dead volume would occur.
31. If a dead volume occurs, stop the pump and repeat step 28 to 30.
32. If no dead volume occurs, the pilot column is released Chapter 6.3, S. 27.
33. Remove liquid above the connecting flange through the two holes in the
adjustable end piece.
YMC recommends flushing this area if high salt concentrations are used.
34. Equilibrate the pilot column with the desired eluent and test the column
performance.
