YMC Europe GmbH
Cleaning and maintencance
YMC Pilot
32 / 49
PLUS
low pressure chromatography column
Cleaning methods for CIP
Treatment
Type of
contamination
Sterilisation Depyrogenation
Pepsin, pH 1.5 - 2
Proteolysis of adsorbed
proteins
No
No
Non-ionic detergents (Triton X-
100; Tween 80)
Removal of hydrophobic
proteins and lipids
No
No
Cationic detergents, pH 9 - 11
Removal of hydrophobic
proteins and lipids
No
Partly
Urea, 6 - 8 M
Removal of protein
aggregates
No
Unknown
1 - 100 mM EDTA, pH
depending on the stationary
phase
Removal of metal
complexes
No
No
0.1 - 1 M NaOH
Removal of bounded
hydrophobic proteins,
lipopolysaccharides
Good
Good
0.5 - 1 M acetic acid in 60%
ethanol
Removal of lipids,
pigments,
lipopolysaccharides, and
other lipophilic
substances
Very good
Good
7.2
Cleaning and maintenance plan
The specified maintenance plan here is to be regarded as a general recommendation.
Depending on the frequency of use and specific application, the appropriate
maintenance plan and the intervals must be defined locally.
Interval
Maintenance
After every operation
• Clean the pilot column.
Daily
• Check the column bed, adjust the piston if necessary.
• Check all tubing and connections regarding leakage Chapter 5.2,
• Fix loosened fixing screws again or replace tubing and connectors.
Every 3 months
• Check the profile gasket in the adjustable end piece.
If damaged, replace it.
• Check the ring gasket of the column body.
If damaged, replace it.
Every 6 months
• Check the frit in the adjustable end piece.
If damaged, replace it Chapter 7.4.1, S. 36.
• Check the frit in the column body.
If damaged, replace it Chapter 7.4.3, S. 40.
