YMC Europe GmbH
Cleaning and maintencance
YMC Pilot
31 / 49
PLUS
low pressure chromatography column
7.1
Notes for Cleaning-In-Place (CIP)
CAREFUL
Packing material
Eye and skin injuries possible through contact with packing material.
► Wear the prescribed personnel protective equipment.
ATTENTION
Cleaning with inappropriate solvents
Material damage possible due to corrosion, leaching or deformation.
► Check the chemical resistance of the wetted parts for the cleaning method before
the CIP procedure.
With the cleaning-in-place procedure, a packed pilot glass column can be cleaned
without unpacking or dismantling. Depending on the contamination, three different
procedures can be used:
1. Regeneration,
2. Sterilisation,
3. Depyrogenation.
Regeneration
Regeneration removes inorganic and organic contamination, which binds to the
chromatography matrix and substantially affects the capacity and resolution of the
column. Commonly these are: lipids, pyrogens, protein aggregates, pigments,
polyphenols or metal complexes.
Sterilisation
Sterilisation is the removal and/or destruction of microorganisms and spores that will
contaminate the purified product. The most common method is the treatment with
sodium hydroxide, acetic acid or ethanol solution.
Depyrogenation
Depyrogenation includes the removal of endotoxins, which can be bound on the
chromatographic material or the column hardware (frits, tubing, etc.) and which can
contaminate the purified product.
