YMC Europe GmbH
Requirements for operation
YMC Pilot
17 / 49
PLUS
low pressure chromatography column
Standard procedure pressure test
1 Pilot column
4 Pressure gauge
2 Back pressure regulator
5 Gate valve
3 Gate valve
6 Pump
The pressure test should be performed as follows:
1. Assemble the system according to the schematic overview of a pressure test.
2. Turn the piston of the adjustable end piece in the DOWN direction as far as it
will go.
3. Fill the pilot column with isopropanol.
4. Increase the testing pressure in the pilot column by slow and stepwise
adjustment of the flow rate at the pump.
The testing pressure is the pressure limit of the column plus 10% (70
mm ID = 17.6 bar | 100 mm ID = 11 bar | 140 mm ID = 7.7 bar | 200
mm ID = 5.5 bar)
5. Keep the testing pressure constant for 10 minutes.
6. If leakages occur, check the connections. If required, replace tubing and/or
connectors.
If the testing pressure was constant for 10 minutes, the column is ready for use.
If a back pressure regulator is used, the maximum pressure is reached
easier.
Leakages at the piston of the adjustable end piece are often related to
impurities trapped in the seal.
