YMC Europe GmbH
Operation
YMC Pilot
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PLUS
low pressure chromatography column
6.2.3
Slurry column packing with sedimentation
The column is packed via the sedimentation slurry method as follows:
1. Remove the adjustable end piece Chapter 6.1, S. 18.
2. Check the drainage tubing for correct connection.
3. Check the drainage tubing outlet for correct position in the collecting vessel.
4. Connect the pump to the tubing of the column body.
5. Start the pump.
6. Fill the glass body with solvent or buffer until 2-3 cm above the frit in the
column body (bottom-up).
This ensures that no air is trapped under or is inside the frit.
7. Close the valve in the tubing of the column body.
8. Disconnect the pump from the column body.
9. Connect the pump to the adjustable end piece.
10. Flush the piston of the adjustable end piece with slurry solvent until the frit in
the adjustable end piece is wetted completely.
11. Disconnect the pump from the adjustable end piece.
12. Pour the homogenous and/or degassed slurry into the glass body.
13. Rinse the inner wall of the column body with slurry solvent in order to remove
particles.
By removing particles from the inner wall of the glass body, a correct seal with
the profile gasket is guaranteed.
14. Do not move the pilot column anymore.
YMC recommends covering the open column body during sedimentation with
some plastic film.
15. Wait until the packing material has settled (sedimentation).
Depending on the packing material, this can take some hours.
16. Fill the pilot column up to the edge of the drain function with slurry solvent.
17. Turn the closing disc of the adjustable end piece in the UP direction as far as it
will go.
