YMC Pilot PLUS, Manual

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YMC Europe GmbH Troubleshooting
YMC Pilot 42 / 49
PLUS
low pressure chromatography column
8 Troubleshooting
WARNING
Incorrect maintenance
Severe injuries during operation after incorrect maintenance possible if the column or
column parts do not withstand the operating pressure.
► Only authorised and qualified personnel should carry out the maintenance.
WARNING
Incorrect replacement of spare parts
Severe injuries due to breakage possible, if not authorised spare parts are used.
► Use only the manufacturer authorised spare parts.
Fault description Possible cause Solution
Dead volume occurred Exceeding the maximum
allowable pressure of the
column packing
► If dead volume occurs, proceed as
follows:
1. Stop the pump.
3. Readjust the piston of the
adjustable end piece.
Stressed column packing
Peak shape of the eluted
substances deteriorates Dead volume at the column
inlet See fault description
“Dead volume
occurred”
Partially clogged inlet frit Frit replacement Chapter 7.4.1, S. 36
Partially clogged outlet frit Frit replacement Chapter 7.4.3, S. 40
Separation performance of
the stationary phase altered
by impurities Wash the stationary phase. Contact
also the manufacturer of the packing
material Chapter 7.1, S. 31
Stationary phase
mechanically destroyed Replace packing material
Air in the column Degassing or evaporation of
solvent during storage New column conditioning. Possibly
new packing material is necessary.
Exceptional pressure rise
during operation Valve circuit incorrect Check valve positions
Clogged frit Frit replacement Chapter 7.4, S. 36
Over-tightened fixing
screws Replace fixing screws and clamping
tubing, cut the end of the tubing
Pressure drop during
operation Leakage of tubing or fitting
between pump and column Check tubing and connections
Depleted solvent reservoir Refill solvents