Figure 16–9: Installation of flow cell
WORK, AUX, and REF are connected using the red, blue, and black cell cables.
Note:
The LC outlet is placed on top, to prevent entrapment of bubbles.
Warning:
Use proper eye and skin protection when working with solvents.
To install the flow cell with salt bridge REF:
1. Inspect the REF for air bubbles and saturation with KCl. Some KCl crystals should be
visible. When no crystals are visible, or when air bubbles are trapped, the REF requires
maintenance. To prevent the REF from drying out, it is sealed with a cap on arrival.
Remove the cap.
2. Tighten the black swivel of the REF. A small droplet should appear at the cotton-wool frit.
Do not remove this droplet because it ensures proper contact of the REF with the mobile
phase.
3. Turn on the HPLC pump. Place some tissues because mobile phase may spill during this
mounting procedure. Connect the column outlet to the flow cell inlet, using small-bore
PEEK tubing (0.3-mm ID) and one of the finger-tights supplied. Use only the factory-
supplied finger-tights in the flow cell; others may cause serious damage. Let the tubing
protrude approximately 1.5 cm from the finger-tight and tighten it carefully.
Note:
Over-tightening affects the flow through the tubing (turbulence) and decreases flow
cell performance.
December 16, 2021, 715007395 Ver. 00
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