hardware are prepared for more than one sensor board. Typically, a two-cell configuration can be
used for detection in serial or parallel mode.
5.4.1 Serial mode detection
In serial mode, one LC system is used with two flow cells in series. For data acquisition, two data
channels are applied with the same time base. Serial mode detection is especially suitable for
OX-RED or RED-OX applications—examples are analysis of vitamin K and nitrotyrosine—using
micro HPLC. The first flow cell is a FlexCell that converts the analyte of interest in a detectable
substance. The second flow cell is a SenCell or VT-03 cell that is used for detection. Note that it
is necessary to work with micro HPLC because the conversion rate of the reactor cell is too low
when using standard HPLC.
Figure 5–3: Typical configuration for serial mode detection: Cell 1 is a FlexCell; cell 2 is a
SenCell for detection; Empower channel 1 and 2 use the same time base as system 1
Degasser
Pump 1
Inject 1
Column 1
Cell 1 Cell 2
3465 Detector
with
Dual Cell Control
Empower Chromatography
Software
Empower instrument 1
Channel 2
Empower instrument 1
Channel 1
5.4.2 Navigation in dual cell menu
All menus for a dual flow cell system are similar to those for a single cell system, with two
exceptions. First, in the top-right corner, a number indicates the active cell. Toggle with the “+”
and “-“ buttons between sensor boards. If the board number does not change, it means that the
second sensor board is not installed or not properly recognized. Second, a new status screen is
available in dual cell systems that indicates the status of both cells in a single screen. However,
for convenience, it is best to use PC control from the Empower software.
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