14.3.1 Connecting a FlexCell to an LC system
To connect a FlexCell to an LC system:
1. Install a suitable length of sharply cut 1/16-inch-OD PEEK tubing to the column outlet.
Choose a tubing ID that matches the column ID:
• 0.25-mm ID for working with normal-bore columns
• 0.13-mm ID for working with mini-bore columns
• 64-µm ID for narrow-bore columns
2. Keep some tissues close by because you will probably spill some mobile phase during the
mounting procedure. Remove the reference electrode from the inlet block (
).
3. With the mobile phase running from the column, connect the column outlet to the flow cell
inlet, using one of the supplied connectors, and tighten it carefully. Over-tightening affects
the flow through the tubing and decreases flow cell performance.
Notice:
Use only the factory-supplied finger-tight connectors at the flow cell;
other connectors may cause serious damage.
4. Install a suitable length of sharply cut 0.5-mm-ID PEEK tubing to the flow cell outlet.
5. For a salt bridge reference electrode, first make sure it is ready for use:
Notice:
When using a salt bridge reference electrode, inspect it visually before
installation:
• No air bubbles trapped in the body
• Visual presence of salt crystals in the body
• The cotton tip looks white and wet
Remove the salt bridge storage cap and tighten the black swivel a little bit extra, which
should result in a small droplet appearing from the frit. This droplet indicates that the frit is
not dried out or clogged. Leave the droplet on the tip when inserting it into the reference
chamber; it ensures proper contact of the REF with the mobile phase.
6. Close the outlet tubing (by finger) and force the running mobile phase into the reference
electrode reservoir.
7. If air bubbles are visibly stuck in the reservoir, remove them with a plastic pipette.
8. Install the reference electrode in the reservoir (without trapping bubbles).
9. Place the cell at an angle of 45° in the detector oven compartment, with the outlet facing
upward, and connect the cell cable as illustrated below.
December 16, 2021, 715007395 Ver. 00
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