Tissue-Tek VIP 6 AI, Operating Manual, Page: 55 / 98

3. CUSTOMIZATION OF SETTINGS
53
To perform a Paraffin Drain:
1.
Touch
Station
button to select the paraffin station to
drain.
WARNING: EMPTY PARAFFIN WASTE
RESERVOIR AND REPLACE THE PARAFFIN
WASTE INSERT (SEE SECTION 2 FOR
INSTRUCTIONS).
2.
Touch View as a List to view as a list.
3. Touch Start to begin the process.
4.
Touch Exit to return to the process menu.
To perform a Reservoir Fill:
1.
Select the bulk reservoir to fill.
2.
Connect external tube to the appropriate bulk reservoir
external fill port.
3.
Touch View as a list to view as a list.
4. Touch Start to begin the process.
5.
Touch
Exit
to return to the process menu.
To create a Defatting Solution:
Defatting solution is used in the defatting step. Perform the
"Defatting solution" operation under the solution exchange
function, and you can automatically fill an empty solution
bottle from the bulk reservoirs to prepare a mixed solution
(Figure 3-CCCC).
The solution name "Ethanol/Xylene (2:1)" indicates a
defatting solution whose mixing ratio is fixed to "alcohol 2 :
xylene 1" (alcohol 2.7 L : xylene 1.5 L). Similarly, the solution
name "Ethanol/Xylene (1:2)" indicates a defatting solution
whose mixing ratio is fixed to "alcohol 1 : xylene 2" (alcohol
1.5 L : xylene 2.7 L).
NOTE: The mixing ratio 2:1 or 1:2 is a rough figure
and not an accurate mixing ratio. In a mixed
solution group, those stations that are recognized
as containing a defatting solution are not subject to
automatic transfer
NOTE: A mixed solution is not automatically
prepared and supplied for automatic transfer
(normally performed for solution groups only) or
during tissue processing, but it is supplied only from
other stations or from the linked bulk reservoir,
which means that the mixing ratio is subject to
change. A mixed solution is also excluded from the
scope of exchange solution functions targeting
solution groups (external drain, external fill, fill
from bulk reservoir).
Figure 3-CCCC
1. Select the designated station to fill with the mixed
reagent.
2. Use the fill quantity selection buttons to select the
appropriate quantity of solution to be filled from bulk
reservoir (Station 19) and bulk reservoir (Station 20).
CAUTION: Both of the bulk reservoirs
require that solution is filled to the Low level
or above. The bulk reservoirs cannot be
selected if the solution level is below Low.
With the bulk reservoir for 2.7-L fill, up to three
stations can be selected if the bulk reservoir
contains solution to the High level. Confirm
that the station configuration includes one of
the special solution names for defatting
solutions. If not, a mixed solution cannot be
prepared.
3. Confirm that the designated solution bottle is empty.
4.
Touch Start to begin the process.
CAUTION: If the conditions are not met, a
cautionary message appears inside the box
on the screen (Figure 3-DDDD). If the solution
bottle is not empty, pump out the solution from
the retort and then end the preparation of
defatting solution.